公开(公告)号：US20210332348A1

公开(公告)日：2021-10-28

申请号：US17237495

申请日：2021-04-22

申请人： Capitalbio Corporation ,  West China Hospital of Sichuan University

发明人： Xiang CHEN ,  Lei WANG ,  Su LI ,  Longtang ZHENG ,  Yanan WANG ,  Fei WEN ,  Juan XIN ,  Wentian ZHANG ,  Jing CHENG

IPC分类号： C12N15/10 ,  C12Q1/6806

摘要： A nucleic acid extraction composition, reagents and kits containing the same and uses thereof. Provided is a nucleic acid extraction and purification reagent free of volatile organic solvents, which prevents the damage of volatile organic solvents to people and greatly improves the timeliness of nucleic acid extraction and purification, making the operation extremely simple, and the nucleic acid can be obtained within 10 minutes. The obtained nucleic acid may be used for biological reactions such as PCR, NASBA, LAMP and RPA. Moreover, the reagents of the present disclosure may be used to extract nucleic acids of cells, bacteria, fungi, DNA viruses and RNA viruses from various samples such as blood, throat swab preserving fluid, saliva, urine, sputum, excrement and the like, and very suitable for clinical and scientific research uses.