Methods related to modified glycans
    1.
    发明授权
    Methods related to modified glycans 有权
    与改性聚糖相关的方法

    公开(公告)号:US09103821B2

    公开(公告)日:2015-08-11

    申请号:US13140553

    申请日:2009-12-18

    摘要: Among other things, the present disclosure provides methods for enriching, identifying, and/or quantifying unusually modified glycans (e.g., phosphorylated glycans, sulfated glycans, and/or multi-acetylated glycans). In many embodiments, methods comprise providing a glycan preparation from which sialic acids have been released; subjecting the sialidase-treated glycan preparation to a separation technique that separates glycans based on charge-to-mass ratio; and quantifying the charged products using at least one quantification standard.

    摘要翻译: 除其他之外,本公开提供了用于富集,鉴定和/或定量异常修饰的聚糖(例如磷酸化聚糖,硫酸化聚糖和/或多乙酰化聚糖)的方法。 在许多实施方案中,方法包括提供已经从其释放唾液酸的聚糖制剂; 将经唾液酸酶处理的聚糖制剂进行基于电荷与质量比的分离聚糖的分离技术; 并使用至少一个定量标准量化带电产物。

    METHODS RELATED TO MODIFIED GLYCANS
    2.
    发明申请
    METHODS RELATED TO MODIFIED GLYCANS 有权
    与修饰的甘蓝相关的方法

    公开(公告)号:US20110306075A1

    公开(公告)日:2011-12-15

    申请号:US13140553

    申请日:2009-12-18

    IPC分类号: C12Q1/34

    摘要: Among other things, the present disclosure provides methods for enriching, identifying, and/or quantifying unusually modified glycans (e.g., phosphorylated glycans, sulfated glycans, and/or multi-acetylated glycans). In many embodiments, methods comprise providing a glycan preparation from which sialic acids have been released; subjecting the sialidase-treated glycan preparation to a separation technique that separates glycans based on charge-to-mass ratio; and quantifying the charged products using at least one quantification standard.

    摘要翻译: 除其他之外,本公开提供了用于富集,鉴定和/或定量异常修饰的聚糖(例如磷酸化聚糖,硫酸化聚糖和/或多乙酰化聚糖)的方法。 在许多实施方案中,方法包括提供已经释放唾液酸的聚糖制剂; 将经唾液酸酶处理的聚糖制剂进行基于电荷与质量比的分离聚糖的分离技术; 并使用至少一个定量标准量化带电产物。