公开(公告)号：US20060166349A1

公开(公告)日：2006-07-27

申请号：US10526089

申请日：2003-06-17

申请人： Cecilia Kepka ,  Jenny Rhodin ,  Folke Tjerneld

发明人： Cecilia Kepka ,  Jenny Rhodin ,  Folke Tjerneld

IPC分类号： C12N15/63

CPC分类号： C12N15/1006

摘要： The present invention is a method for the purification of plasmid DNA comprising to provide a composition comprising a first polymer having inverse solubility characteristics and a second polymer immiscible in the first polymer; contacting said solution with an aqueous solution comprising plasmid DNA; providing phase separation and isolating the aqueous phase; and increasing the temperature of the isolated phase to a temperature above the cloud point of the first polymer and below the temperature where plasmid DNA is degraded and subsequently isolating the aqueous phase so formed. The invention also encompasses a kit for purification of plasmid DNA as described above.