公开(公告)号：US20190024065A1

公开(公告)日：2019-01-24

申请号：US15546926

申请日：2016-02-05

Applicant: Cellectis

Inventor： Fabien DELACOTE ,  Philippe DUCHATEAU

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N5/0789

Abstract: The present invention relates to a non-viral method for transfecting a hematopoietic cell which can be employed in immunotherapy. This method is based on the use of nanoparticle-biomolecule conjugates with increased homologous recombination. Nucleic acid to be transfected can be a chimeric antigen receptor and/or encoding a target-specific endonuclease. The present invention relates also to a method for transfecting APCs. Furthermore, the present invention relates to pharmaceutical compositions, uses and kits.