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    Method to perform intrinsic hyper-spectral imaging
    1.
    发明授权
    Method to perform intrinsic hyper-spectral imaging 有权

    公开(公告)号：US12106520B1

    公开(公告)日：2024-10-01

    申请号：US18424779

    申请日：2024-01-27

    Applicant: Center for Quantitative Cytometry

    Inventor： Abraham Schwartz ,  Philip Sherman

    IPC: G06T7/80 ,  G01J3/28

    CPC classification number: G06T7/80 ,  G01J3/2823 ,  G01J2003/2826 ,  G01J2003/2879 ,  G06T2207/10032 ,  G06T2207/20224

    Abstract: A method to obtain and process data to generate intrinsic hyper-spectral data cubes is provided, where a data acquisition procedure involves scanning the field of view under focused and diffused conditions and an intrinsic calibration procedure requires focused and diffused scans of the field of view of a white reference surface. The spectra of the diffuse scan of the white reference surface is subtracted from the spectra of the focused scan of the white reference surface resulting in a residual data cube of a scan of the white surface. To obtain an intrinsic data cube of a field of view of interest, the residual data cube is added to the diffused data cube of the field of view of interest generating a resulting data cube that is subtracted from the focused data cube field of view of interest.

    Method to obtain data cubes of low intensity intrinsic spectral images when imaging under high illumination conditions without the use of filters
    2.
    发明授权
    Method to obtain data cubes of low intensity intrinsic spectral images when imaging under high illumination conditions without the use of filters 有权

    公开(公告)号：US10652484B1

    公开(公告)日：2020-05-12

    申请号：US16657937

    申请日：2019-10-18

    Applicant: Center for Quantitative Cytometry

    Inventor： Abraham Schwartz ,  Philip Sherman ,  Adolfas Gaigalas ,  Peter Ingram

    IPC: H04N5/353 ,  H04N5/232 ,  H04N5/235 ,  G01J3/02 ,  G06T7/80 ,  H04N5/33

    Abstract: The invention provides a method of obtaining data cubes such that elimination of the irrelevant portion of the illumination reveals the low intensity intrinsic spectral components. The method obtains target and reference data cubes using a single camera and of a single field of view so that low-level intrinsic spectral components may be detected and enhanced under conditions of high illumination intensity without the use of filters. Two exposures in rapid succession of the same field of view at the same camera settings are taken, one exposure of the target is taken under coherent conditions where the field of view is in focus, and the second exposure is of the same field of view taken under non-coherent conditions where the target is defocused such that it has no discernible spatial features in the field of view. Residual spectra previously determined is added to the defocus spectra in the defocus data cube of the target of interest resulting in an adjusted reference spectra, which in turn is subtracted from the corresponding focused spectra in the focused data cube of the target of interest resulting in a focused target data cube each of whose pixels is associated with its intrinsic spectrum.

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