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公开(公告)号：US20140242579A1
公开(公告)日：2014-08-28
申请号：US13952377
申请日：2013-07-26
发明人： Wei ZHOU , Glenn MCGALL , Moti JAIN , Rui MEI
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C07H19/10 , C07H19/20 , C07H21/00
摘要： The present invention relates generally to methods of sequencing of polynucleotides and compounds, compositions and kits useful for sequencing of polynucleotides. The chemical compounds include nucleotide and nucleoside analogs which possess a blocking group covalently attached to the 3′ hydroxyl of the sugar moiety. The blocking group may optionally be additionally covalently attached to a linker and/or detectable label. The nucleotide analogs may be ribonucleotide or deoxyribonucleotide analogs. Methods include incorporation of the reversible terminator molecules into growing polynucleotide strands by polymerase enzymes, such as in single base sequencing methodologies utilizing sequential reversible termination techniques.
摘要翻译： 本发明一般涉及多核苷酸和化合物的测序方法，可用于多核苷酸测序的组合物和试剂盒。 化合物包括具有与糖部分的3'羟基共价连接的封闭基团的核苷酸和核苷类似物。 封闭基团可以任选另外共价连接到接头和/或可检测标记上。 核苷酸类似物可以是核糖核苷酸或脱氧核糖核苷酸类似物。 方法包括通过聚合酶将可逆终止子分子掺入生长的多核苷酸链中，例如使用顺序可逆终止技术的单碱基测序方法。
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公开(公告)号：US20170218441A1
公开(公告)日：2017-08-03
申请号：US15481194
申请日：2017-04-06
发明人： Wei ZHOU , Glenn MCGALL , Moti JAIN , Rui MEI
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C07H19/10 , C07H19/20 , C07H21/00
摘要： The present invention relates generally to methods of sequencing of polynucleotides and compounds, compositions and kits useful for sequencing of polynucleotides. The chemical compounds include nucleotide and nucleoside analogs which possess a blocking group covalently attached to the 3′ hydroxyl of the sugar moiety. The blocking group may optionally be additionally covalently attached to a linker and/or detectable label. The nucleotide analogs may be ribonucleotide or deoxyribonucleotide analogs. Methods include incorporation of the reversible terminator molecules into growing polynucleotide strands by polymerase enzymes, such as in single base sequencing methodologies utilizing sequential reversible termination techniques.
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公开(公告)号：US20180010180A1
公开(公告)日：2018-01-11
申请号：US15631414
申请日：2017-06-23
发明人： Wei ZHOU , Rui MEI , Hajime MATSUZAKI
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2525/185 , C12Q2527/113 , C12Q2535/122 , C12Q2521/501 , C12Q2525/186 , C12Q2535/101
摘要： The present invention provides methods and systems for sequencing long nucleic acid fragment. The present invention also provides a method of sequencing a target polynucleotide with fewer probes. Further, the present invention provides a method of sequencing a target polynucleotide with longer reads. Locus-specific, ligation-assisted sequencing/genotyping method and ligation-captured sequencing method are also provided in the present invention. The methods of the present invention allow low-cost, high-throughput and accurate sequencing of nucleic acids.
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公开(公告)号：US20210180123A1
公开(公告)日：2021-06-17
申请号：US17005496
申请日：2020-08-28
发明人： Wei ZHOU , Rui MEI , Filip CRNOGORAC , Guochun LIAO , Julian LUCAS
IPC分类号： C12Q1/6869 , C12Q1/6874
摘要： The disclosure provides methods and systems for sequencing long nucleic acid fragments. In one aspect, methods, systems and reagent kits are provided for sequencing nucleic acid target sequences. Some embodiments of the methods, systems and reagent kits are particularly suitable for sequencing a large number of fragments, particularly long fragments.
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