公开(公告)号：US11993815B2

公开(公告)日：2024-05-28

申请号：US17392175

申请日：2021-08-02

申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

发明人： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6876

CPC分类号： C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6869 ,  C12Q2525/179 ,  C12Q2525/185 ,  C12Q2525/191 ,  C12Q2535/119 ,  C12Q1/6806 ,  C12Q2525/191 ,  C12Q2535/119 ,  C12Q2535/122 ,  C12Q2563/179 ,  C12Q2565/514

摘要： Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

公开(公告)号：US10077474B2

公开(公告)日：2018-09-18

申请号：US13840142

申请日：2013-03-15

申请人： Abbott Molecular Inc.

发明人： Shihai X. Huang ,  Hong X. Su ,  Brian J. Erickson

IPC分类号： C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q1/6858

CPC分类号： C12Q1/6876 ,  C12Q1/6858 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2525/185 ,  C12Q2535/125

摘要： A method of designing a primer for detecting a single nucleotide polymorphism (SNP), a method of detecting an SNP, a method of distinguishing SNPs, primers, detectable oligonucleotides, and kits.

公开(公告)号：US20180037951A1

公开(公告)日：2018-02-08

申请号：US15552040

申请日：2016-02-19

申请人： The Regents of the University of California

发明人： Sriram Kosuri ,  Rocky Cheung ,  Nathan B. Lubock

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6874 ,  C12N15/1065 ,  C12N15/1082 ,  C12Q1/6869 ,  C12Q2521/507 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/185 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2531/125

摘要： In some aspects, the invention relates to a method for producing a barcoded DNA library, comprising: optionally fragmenting DNA to produce fragmented DNA; combining the DNA/fragments with control nucleotide sequences to produce a mixture of nucleic acids, each control nucleotide sequence comprising a variable nucleotide sequence, thereby barcoding the DNA/fragments; amplifying the mixture of nucleic acids to produce amplified nucleic acids; isolating a plurality of nucleic acids from the amplified nucleic acids; and rearranging the nucleic acids of the plurality, thereby producing the barcoded DNA library.

公开(公告)号：US09856521B2

公开(公告)日：2018-01-02

申请号：US14788670

申请日：2015-06-30

申请人： BioSpyder Technologies, Inc.

发明人： Anthony Stevens ,  Bruce Seligmann ,  Joanne M. Yeakley ,  Joel McComb

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： C12Q1/6827 ,  C12Q2521/325 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/185 ,  C12Q2525/186 ,  C12Q2533/101 ,  C12Q2533/107 ,  C12Q2535/122 ,  C12Q2565/629 ,  C12Q2521/307 ,  C12Q2561/125

摘要： Ligation assays in liquid phase for detecting nucleic acid sequences.

公开(公告)号：US20170247747A1

公开(公告)日：2017-08-31

申请号：US15517042

申请日：2015-10-09

申请人： Rutgers, The State University of New Jersey

发明人： David Alland ,  Soumitesh Chakravorty

IPC分类号： C12Q1/68 ,  G01N33/50

CPC分类号： C12Q1/689 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q2525/143 ,  C12Q2525/185 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/5008

摘要： The present invention relates to novel primers and sloppy molecular beacon and molecular beacon probes for amplifying segments from different genes in Mycobacterium tuberculosis for identifying the presence of M.tb DNA and/or resistance to anti-tuberculosis drugs.

公开(公告)号：US09689032B2

公开(公告)日：2017-06-27

申请号：US13970321

申请日：2013-08-19

申请人： Centrillion Technology Holdings Corporation

发明人： Wei Zhou ,  Rui Mei ,  Filip Crnogorac ,  Guochun Liao ,  Julian Lucas

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/185 ,  C12Q2527/113 ,  C12Q2535/122

摘要： The present invention provides methods and systems for sequencing long nucleic acid fragments.

公开(公告)号：US20170101671A1

公开(公告)日：2017-04-13

申请号：US15387650

申请日：2016-12-22

申请人： BioSpyder Technologies, Inc.

发明人： Anthony Stevens ,  Bruce Seligmann ,  Joanne M. Yeakley ,  Joel McComb

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  C12Q2521/325 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/185 ,  C12Q2525/186 ,  C12Q2533/101 ,  C12Q2533/107 ,  C12Q2535/122 ,  C12Q2565/629 ,  C12Q2521/307 ,  C12Q2521/319 ,  C12Q2525/197 ,  C12Q2525/207 ,  C12Q2561/125 ,  C12Q2563/149

摘要： Ligation assays in liquid phase for detecting nucleic acid sequences.

公开(公告)号：US09605302B2

公开(公告)日：2017-03-28

申请号：US13062213

申请日：2009-09-03

申请人： Jonathan O'Halloran

发明人： Jonathan O'Halloran

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6825 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2565/631 ,  C12Q2525/204 ,  C12Q2525/131 ,  C12Q2525/197 ,  C12Q2525/185 ,  C12Q2525/161

摘要： Embodiments of the present invention relate generally to strategies and methods of amplifying short target sequences and removing flanking sequences from target nucleic acids to remove background signal when detecting hybridizations events using sensitive detection biosensors, such as biosensors based on nanowires, carbon nanotubes, nanopores etc, that may be capable of detecting molecules at small molar concentrations (fM and less), or even at the single molecule level. Furthermore, by cropping and therefore standardizing the size of the target sequences to be detected, when detecting many target sequences in an array, the signals across each biosensor can be compared and the hybridization conditions standardized easily.

公开(公告)号：US20160222447A1

公开(公告)日：2016-08-04

申请号：US14788670

申请日：2015-06-30

申请人： Anthony Stevens ,  Bruce Seligmann ,  Joanne M. Yeakley ,  Joel McComb

发明人： Anthony Stevens ,  Bruce Seligmann ,  Joanne M. Yeakley ,  Joel McComb

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  C12Q2521/325 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/185 ,  C12Q2525/186 ,  C12Q2533/101 ,  C12Q2533/107 ,  C12Q2535/122 ,  C12Q2565/629 ,  C12Q2521/307 ,  C12Q2561/125

摘要： Ligation assays in liquid phase for detecting nucleic acid sequences.

摘要翻译： 用于检测核酸序列的液相中的连接测定。

公开(公告)号：US09249458B1

公开(公告)日：2016-02-02

申请号：US14524227

申请日：2014-10-27

申请人： Steven Albert Benner

发明人： Steven Albert Benner

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H19/04 ,  C07H21/04

CPC分类号： C12Q1/6853 ,  C07H21/04 ,  C12Q1/6848 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2600/16 ,  C12Q2531/113 ,  C12Q2527/107 ,  C12Q2525/185 ,  C12Q2537/143 ,  C12Q2525/101

摘要： This invention combines artificially expanded genetic information systems (AEGIS) with self-avoiding molecular recognition systems (SAMRS), in processes that involve template-directed primer extension in highly multiplexed form in mixtures containing large numbers of primers. This process yields extension products, or in its PCR format, amplicons, that have AEGIS tags that can be cleanly captured in highly complex mixtures.

摘要翻译： 本发明将人为扩增的遗传信息系统（AEGIS）与自我回避分子识别系统（SAMRS）结合，在包含大量引物的混合物中涉及以高度多重化形式进行模板定向引物延伸的方法。 该方法产生扩增产物或其PCR格式的扩增子，其具有可以在高度复杂的混合物中被干净捕获的AEGIS标签。