公开(公告)号：US20240263148A1

公开(公告)日：2024-08-08

申请号：US18005027

申请日：2021-11-23

申请人： Cevec Pharmaceuticals GmbH

发明人： Juliana Coronel ,  Silke Wissing

IPC分类号： C12N7/00 ,  C12M1/00 ,  C12N15/86

CPC分类号： C12N7/00 ,  C12M29/10 ,  C12N15/86 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152

摘要： The present invention relates to methods for the production of Adeno-associated virus (AAV), comprising steps of providing a stable AAV producer cell line in which at least some or all genes encoding the components necessary for the production of AAV are stably integrated into the cell genome, and culturing said cells in perfusion culture during the AAV production step (i.e., during the N step), wherein said perfusion culture encompasses continuous replacement of spent media with fresh media, and wherein said continuous replacement of spent media with fresh media continues after the induction of AAV production.