公开(公告)号：US20190170745A1

公开(公告)日：2019-06-06

申请号：US16322014

申请日：2017-07-28

Applicant: Che-Ming Jack HU ,  ACADEMIA SINICA

Inventor： Che-Ming Jack HU ,  Hui-Wen CHEN ,  Yuan-I CHEN ,  Chen-Ying CHEIN ,  Jung-Chen LIN

IPC: G01N33/556 ,  G01N33/543 ,  C12N5/078

Abstract: A method of generating an internally fixed lipid vesicle, comprising: providing a precursor lipid vesicle that contains an aqueous interior enclosed by a lipid membrane, wherein the lipid membrane of the precursor lipid vesicle is non-permeable to a crosslinker; permeabilizing the lipid membrane transiently to generate a permeable vesicle; contacting the permeable vesicle with an inactive activatable crosslinker, whereby the inactive activatable crosslinker enters the permeable vesicle; allowing the permeable vesicle to return to a non-permeable vesicle; removing any extravesicular crosslinker; and activating the inactive activatable crosslinker to allow crosslinking to occur inside the non-permeable vesicle, whereby an internally fixed lipid vesicle is generated.