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公开(公告)号:US20190170745A1
公开(公告)日:2019-06-06
申请号:US16322014
申请日:2017-07-28
Applicant: Che-Ming Jack HU , ACADEMIA SINICA
Inventor: Che-Ming Jack HU , Hui-Wen CHEN , Yuan-I CHEN , Chen-Ying CHEIN , Jung-Chen LIN
IPC: G01N33/556 , G01N33/543 , C12N5/078
Abstract: A method of generating an internally fixed lipid vesicle, comprising: providing a precursor lipid vesicle that contains an aqueous interior enclosed by a lipid membrane, wherein the lipid membrane of the precursor lipid vesicle is non-permeable to a crosslinker; permeabilizing the lipid membrane transiently to generate a permeable vesicle; contacting the permeable vesicle with an inactive activatable crosslinker, whereby the inactive activatable crosslinker enters the permeable vesicle; allowing the permeable vesicle to return to a non-permeable vesicle; removing any extravesicular crosslinker; and activating the inactive activatable crosslinker to allow crosslinking to occur inside the non-permeable vesicle, whereby an internally fixed lipid vesicle is generated.