公开(公告)号：US20210239693A1

公开(公告)日：2021-08-05

申请号：US17233859

申请日：2021-04-19

申请人： KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, SEJONG CAMPUS

发明人： Jin Sung PARK ,  Seong Jae JO ,  Joo Hyung PARK ,  Won Seok LEE ,  Heon Jeong LEE ,  Dae Sung YOON ,  Gyu Do LEE ,  In Su KIM

IPC分类号： G01N33/556 ,  G01N33/553 ,  G01N33/86 ,  G01N33/74 ,  G01N27/12

摘要： Provided is a biomaterial detection sensor. The biomaterial detection sensor comprises a substrate and a detection probe including a metal nanoparticle deposited on the substrate, and an erythrocyte membrane conformally covering the metal nanoparticle, wherein the detection probe may selectively react with fibrinogen.

公开(公告)号：US20160266142A1

公开(公告)日：2016-09-15

申请号：US15092499

申请日：2016-04-06

申请人： Theranos, Inc.

发明人： Paul Patel ,  Samartha Anekal ,  Ian Gibbons ,  Elizabeth A. Holmes

IPC分类号： G01N33/68 ,  G01N33/543 ,  G01N33/556

摘要： Methods, compositions, systems, and devices are provided for performing and analyzing agglutination assays. In one aspect, methods for image analysis of agglutination assays are provided. In another aspects, methods for performing agglutination assays are provided. In one aspect, the methods may be used for the detection of various molecules, including viruses or antibodies against a virus. In another aspect, the methods can be used to determine effective immunization of a subject.

公开(公告)号：US20070292839A1

公开(公告)日：2007-12-20

申请号：US10577358

申请日：2004-11-01

申请人： David Parker

发明人： David Parker

IPC分类号： C12Q1/70 ,  G01N33/556

CPC分类号： G01N33/6854 ,  G01N2469/20

摘要： The invention relates to a method of determining the amount or presence of antibodies to an immunogen in a blood sample comprising at least the steps of detecting the amount or presence of plasma antibodies or parts thereof and lymphocyte antibodies or parts thereof in said sample, wherein said plasma and lymphocyte antibodies are detected together or in separate assays and determination of their combined amount or presence determines the amount or presence of antibodies to said immunogen. Uses of the method in high throughput screening, screening of blood bank samples and in diagnosis are also provided.

摘要翻译： 本发明涉及一种确定血液样品中免疫原抗体的量或存在的方法，包括至少检测所述样品中血浆抗体或其部分的量或存在的量以及淋巴细胞抗体或其部分的步骤，其中所述 血浆和淋巴细胞抗体一起检测或在单独的测定中检测，并且其组合量或存在的测定决定了抗体对所述免疫原的量或存在。 还提供了在高通量筛选，血库样本筛选和诊断中使用该方法。

公开(公告)号：US5637472A

公开(公告)日：1997-06-10

申请号：US391869

申请日：1995-02-22

申请人： Edward N. Granados ,  Daniel F. Heiman ,  Herbert S. Chow ,  Maria L. Thaler

发明人： Edward N. Granados ,  Daniel F. Heiman ,  Herbert S. Chow ,  Maria L. Thaler

IPC分类号： C12N5/06 ,  G01N33/50 ,  G01N33/543 ,  G01N33/554 ,  G01N33/556 ,  G01N33/569

CPC分类号： G01N33/554 ,  G01N33/5005 ,  G01N33/54313 ,  G01N33/556 ,  G01N33/56977 ,  Y10T436/101666

摘要： A method is provided for the preparation of semisolid particles or cells that have been chemically derivatized resulting in hydrazide functionalities covalently incorporated onto cell surface membrane molecules of the semisolid particles or cells, as well as a method for determining the levels of hydrazide groups on the particles or cells. The method involves the preparation and analysis of red blood cells chemically derivatized in such a manner that hydrazide functionalities have been covalently incorporated onto the cell membrane molecules, the preparation of hydrazine derivatized cells and the use thereof of the novel cells in agglutination reactions to promote the attachment of antigens and antibodies to the hydrazine derivatized cells without loss of antibody activity or the blocking of the etitope of interest.

摘要翻译： 提供了一种制备半固体颗粒或细胞的方法，该半固体颗粒或细胞被化学衍生化，导致共价掺入到半固体颗粒或细胞的细胞表面膜分子上的酰肼官能团，以及确定颗粒上酰肼基团水平的方法 或细胞。 该方法涉及红细胞的制备和分析，其方法是将酰肼官能团共价掺入到细胞膜分子上，制备肼衍生化细胞，以及在凝集反应中使用新细胞促进 将抗原和抗体连接到肼衍生化的细胞上，而不会损失抗体活性或阻断目的蛋白酶。

公开(公告)号：US5318913A

公开(公告)日：1994-06-07

申请号：US420345

申请日：1989-10-12

申请人： Edgar H. Relyveld

发明人： Edgar H. Relyveld

IPC分类号： A61K39/00 ,  A61K39/44 ,  G01N33/555 ,  G01N33/556 ,  G01N33/569

CPC分类号： G01N33/556 ,  G01N33/56911 ,  Y10S435/967 ,  Y10S435/975 ,  Y10S436/808

摘要： The invention relates to a method of preparing a reagent for the determination by hemagglutination of antibodies to bacterial toxins. According to this method erythrocytes are treated with glutaraldehyde and then with the bacterial toxins in the presence of glutaraldehyde without a wash step. The reagent thus obtained is further treated with a reagent for blocking aldehyde groups.

摘要翻译： 本发明涉及制备用于通过细菌毒素抗体血细胞凝集测定的试剂的方法。 根据该方法，用戊二醛处理红细胞，然后用戊二醛存在下用细菌毒素处理，不用洗涤步骤。 由此获得的试剂进一步用用于封闭醛基的试剂处理。

公开(公告)号：US3987159A

公开(公告)日：1976-10-19

申请号：US447205

申请日：1974-03-01

申请人： Jurgen Spona ,  Michael Topert

发明人： Jurgen Spona ,  Michael Topert

IPC分类号： G01N33/556 ,  G01N33/555 ,  A61K35/12 ,  G01N1/00 ,  G01N31/00 ,  G01N33/16

CPC分类号： G01N33/555 ,  Y10S436/814 ,  Y10S436/817 ,  Y10S436/818 ,  Y10S436/826

摘要： Stable preparations of sensitized erythrocytes are provided by simultaneously admixing washed erythrocytes in a buffered aqueous solution of glutaraldehyde, antigen or antigen-protein conjugate and formaldehyde. After washing, the separated sensitized erythrocytes can be lyophilized and thereby become stable at room temperature.

公开(公告)号：US20150198591A1

公开(公告)日：2015-07-16

申请号：US14598498

申请日：2015-01-16

申请人： Theranos, Inc.

发明人： Paul Patel ,  Samartha Anekal ,  Ian Gibbons ,  Elizabeth A. Holmes ,  Swapna Joshi

IPC分类号： G01N33/556 ,  G01N33/68 ,  G01N33/569

CPC分类号： G01N33/86 ,  B01L3/5021 ,  G01N33/54313 ,  G01N33/556 ,  G01N33/56983 ,  G01N33/6854

摘要： Methods, compositions, systems, and devices are provided for performing and analyzing agglutination assays. In one aspect, methods for image analysis of agglutination assays are provided. In another aspects, methods for performing agglutination assays are provided. In one aspect, the methods may be used for the detection of various molecules, including viruses or antibodies against a virus. In another aspect, the methods can be used to determine effective immunization of a subject.

摘要翻译： 提供方法，组合物，系统和装置用于进行和分析凝集测定。 在一个方面，提供了用于凝集测定的图像分析的方法。 在另一方面，提供了用于进行凝集测定的方法。 在一个方面，所述方法可用于检测各种分子，包括针对病毒的病毒或抗体。 另一方面，该方法可用于确定受试者的有效免疫。

公开(公告)号：US4668637A

公开(公告)日：1987-05-26

申请号：US619467

申请日：1984-05-30

申请人： Jean-Luc Guesdon ,  Stratis Avrameas

发明人： Jean-Luc Guesdon ,  Stratis Avrameas

IPC分类号： G01N33/00 ,  G01N1/28 ,  G01N33/531 ,  G01N33/532 ,  G01N33/543 ,  G01N33/555 ,  G01N33/58 ,  G01N33/80 ,  G01N33/567 ,  G01N33/556

CPC分类号： G01N33/54306 ,  G01N33/532 ,  G01N33/555 ,  G01N33/586 ,  G01N33/80 ,  Y10S436/809 ,  Y10S436/826

摘要： A process for the detection of a biological substance immobilized on a support including(1) incubating, after washing, the substance immobilized on a support with the product of coupling of a specific ligand with a ligand capable of reacting with erythrocytes;(2) adding erythrocytes;(3) immersing the whole in a solution of a fixing agent and(4) measuring the erythroadsorption, after having turned the support over in order to permit the removal of the red cells which have not reacted.

摘要翻译： PCT No.PCT / FR83 / 00198 Sec。 371日期1984年5月30日 102（e）日期1984年5月30日PCT提交1983年9月30日PCT公布。 出版物WO84 / 01436 日本1984年4月12日。一种用于检测固定在载体上的生物物质的方法，包括（1）在洗涤之后，将固定在载体上的物质与特异性配体与能够反应的配体偶联的产物 与红细胞; （2）加入红细胞; （3）将固定剂浸渍在固定剂的溶液中，（4）测定红外吸收后，使载体转动，以便除去未反应的红细胞。

公开(公告)号：US4632907A

公开(公告)日：1986-12-30

申请号：US766940

申请日：1985-08-19

申请人： Akihiko Sato ,  Kunihiro Nakajima

发明人： Akihiko Sato ,  Kunihiro Nakajima

IPC分类号： A01N1/02 ,  A61K35/18 ,  A61K38/16 ,  C12Q1/56 ,  G01N33/555 ,  G01N33/556 ,  G01N33/569 ,  G01N33/92 ,  G01N33/96 ,  G01N31/00

CPC分类号： A01N1/0215 ,  A01N1/02 ,  G01N33/556 ,  Y10T436/101666 ,  Y10T436/104165 ,  Y10T436/105831 ,  Y10T436/106664 ,  Y10T436/108331

摘要： A preservative solution for fixed avian erythrocytes for the viral hemagglutination test of this invention comprises purified water which may contain serum albumin, glycerol or dimethyl sulfoxide and, additionally, a preventive against the growth of bacteria as occasion demands. Fixed avian erythrocytes can stably be preserved in this solution. HA and HI test can precisely be performed by employing fixed avian erythrocytes suspended in this invention.

摘要翻译： 用于本发明的病毒血凝试验的固定禽红细胞的防腐剂溶液包含可以含有血清白蛋白，甘油或二甲基亚砜的纯化水，另外，根据需要防止细菌生长。 固定的禽类红细胞可以稳定地保留在该溶液中。 可以通过使用悬浮在本发明中的固定禽类红细胞来精确地进行HA和HI检验。

公开(公告)号：US4298346A

公开(公告)日：1981-11-03

申请号：US085641

申请日：1979-01-29

申请人： Homu Ito

发明人： Homu Ito

IPC分类号： G01N33/556 ,  G01N33/48

CPC分类号： G01N33/556 ,  Y10S435/962 ,  Y10S436/811

摘要： The nonspecific hemagglutination inhibitors and natural hemagglutinins in a test serum for a virus hemagglutination-inhibition reaction can be removed at one stroke in a convenient procedure by pretreating the test serum with a composition containing both fixed avian erythrocytes and kaolin particles.

摘要翻译： 用于病毒血凝抑制反应的测试血清中的非特异性血凝素抑制剂和天然血凝素可以通过用含有固定的禽类红细胞和高岭土颗粒的组合物预处理测试血清在一个方便的程序中一次除去。