公开(公告)号：US08304210B2

公开(公告)日：2012-11-06

申请号：US12919198

申请日：2009-02-06

申请人： Cheol-Ho Pan ,  Joo Young Lee ,  Byung Hun Um ,  Sang Moo Kim ,  Dae-Geun Song

发明人： Cheol-Ho Pan ,  Joo Young Lee ,  Byung Hun Um ,  Sang Moo Kim ,  Dae-Geun Song

IPC分类号： C12P21/06

CPC分类号： C12N15/70 ,  C07K14/245 ,  C07K2319/02

摘要： The present invention relates to a signal sequence peptide for the improvement of extracellular secretion efficiency of a heterologous protein in E. coli. More particularly, the present invention relates to a gene construct for the improvement of extracellular secretion efficiency of the said heterologous protein in E. coli, which comprises a polynucleotide encoding a recombinant protein composed of the heterologous protein linked to C-terminal of the signal sequence peptide represented by SEQ. ID. NO. 3. The present invention contributes to the improvement of extracellular secretion efficiency of a recombinant protein, so that it can be effectively applied to the production of a recombinant protein.

摘要翻译： 本发明涉及用于提高大肠杆菌中异源蛋白质的细胞外分泌效率的信号序列肽。 更具体地，本发明涉及用于改善大肠杆菌中所述异源蛋白质的细胞外分泌效率的基因构建体，其包含编码由与信号序列的C末端连接的异源蛋白质组成的重组蛋白质的多核苷酸 SEQ ID NO： ID。 没有。 本发明有助于提高重组蛋白的细胞外分泌效率，从而可以有效地应用于重组蛋白的生产。

公开(公告)号：US20110033890A1

公开(公告)日：2011-02-10

申请号：US12919198

申请日：2009-02-06

申请人： Cheol-Ho Pan ,  Joo Young Lee ,  Byung Hun Um ,  Sang Moo Kim ,  Dae-Geun Song

发明人： Cheol-Ho Pan ,  Joo Young Lee ,  Byung Hun Um ,  Sang Moo Kim ,  Dae-Geun Song

IPC分类号： C12P21/06 ,  C12P21/02 ,  C12N9/96

CPC分类号： C12N15/70 ,  C07K14/245 ,  C07K2319/02

摘要： The present invention relates to a signal sequence peptide for the improvement of extracellular secretion efficiency of a heterologous protein in E. coli. More particularly, the present invention relates to a gene construct for the improvement of extracellular secretion efficiency of the said heterologous protein in E. coli, which comprises a polynucleotide encoding a recombinant protein composed of the heterologous protein linked to C-terminal of the signal sequence peptide represented by SEQ. ID. NO. 3. The present invention contributes to the improvement of extracellular secretion efficiency of a recombinant protein, so that it can be effectively applied to the production of a recombinant protein.

摘要翻译： 本发明涉及用于提高大肠杆菌中异源蛋白质的细胞外分泌效率的信号序列肽。 更具体地，本发明涉及用于改善大肠杆菌中所述异源蛋白质的细胞外分泌效率的基因构建体，其包含编码由与信号序列的C末端连接的异源蛋白质组成的重组蛋白质的多核苷酸 SEQ ID NO： ID。 没有。 本发明有助于提高重组蛋白的细胞外分泌效率，从而可以有效地应用于重组蛋白的生产。