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公开(公告)号：US07122320B2
公开(公告)日：2006-10-17
申请号：US09842776
申请日：2001-04-27
申请人： Christian Reiter , Gerhard Cullman , Ulrike Friedrichs , Petra Heppner , Meret Lakner , Achim Ringeis
发明人： Christian Reiter , Gerhard Cullman , Ulrike Friedrichs , Petra Heppner , Meret Lakner , Achim Ringeis
IPC分类号： G01N33/53 , G01N33/537 , G01N33/554 , G01N33/567 , G01N33/569
CPC分类号： G01N33/56922 , C07K16/121 , G01N2333/205
摘要： The invention relates to a method for detecting an infection of a mammal with an acid-resistant microorganism, wherein (a) a stool sample of a mammal is incubated with at least two different monoclonal antibodies, fragments or derivatives thereof or aptamers under conditions allowing a complex formation of antigens of the acid-resistant microorganism with antibodies, fragments or derivatives thereof or the aptamers, and wherein (aa) the first monoclonal antibody or the fragment or the derivative thereof or the first aptamer specifically binds an epitope of the first antigen, which shows at least with some mammals a structure after the intestinal passage that corresponds to the native structure or the structure which a mammal produces antibodies against after being infected or immunised with the acid-resistant microorganism or an extract or lysate thereof or a protein therefrom or a fragment thereof or a synthetic peptide; (ab) the second monoclonal antibody or the fragment or the derivative thereof or the second aptamer specifically binds an epitope of a second antigen differing from the epitope of the first antigen, which shows at least with some mammals a structure after the intestinal passage that corresponds to the native structure or the structure which a mammal produces antibodies against after being infected or immunised with the acid-resistant microorganism or an extract or lysate thereof or a protein therefrom or a fragment thereof or a synthetic peptide, wherein the parts of the mammals may overlap according to (aa) and (ab) and in total essentially make up the overall number of infected mammals; and (b) the formation of at least one antigen-antibody complex or antigen-aptamer complex according to (aa) or (ab) is detected.
摘要翻译： 本发明涉及一种用耐酸微生物检测哺乳动物的感染的方法，其中（a）将哺乳动物的粪便样品与至少两种不同的单克隆抗体，其片段或衍生物或适体在允许 用抗体，其片段或衍生物或适配体复合形成抗酸微生物的抗原，并且其中（aa）第一单克隆抗体或其片段或其衍生物或第一适体特异性结合第一抗原的表位， 其至少与一些哺乳动物一起显示出肠道通过后的结构，该结构对应于哺乳动物产生针对抗酸微生物或其提取物或裂解物或蛋白质的免疫后的抗原的天然结构或结构，或 其片段或合成肽; （ab）第二单克隆抗体或其片段或其衍生物或第二适体特异性结合不同于第一抗原表位的第二抗原的表位，其至少显示出一些哺乳动物在肠道通过后对应的结构 哺乳动物产生抗体的抗原结构或结构，用抗酸微生物或其提取物或裂解物或其蛋白质或其片段或合成肽进行感染或免疫之后产生抗体，其中哺乳动物的部分可以 根据（aa）和（ab）重叠，总共基本上构成感染哺乳动物的总数; 和（b）检测根据（aa）或（ab）形成至少一种抗原 - 抗体复合物或抗原 - 适配子复合物。
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公开(公告)号：US07736859B2
公开(公告)日：2010-06-15
申请号：US11513129
申请日：2006-08-31
申请人： Christian Reiter , Gerhard Cullman , Ulrike Friedrichs , Petra Heppner , Meret Lakner , Achim Ringeis
发明人： Christian Reiter , Gerhard Cullman , Ulrike Friedrichs , Petra Heppner , Meret Lakner , Achim Ringeis
IPC分类号： G01N33/53 , G01N33/537 , G01N33/554 , G01N33/567 , G01N33/569
CPC分类号： G01N33/56922 , C07K16/121 , G01N2333/205
摘要： The invention relates to a method for detecting an infection of a mammal with an acid-resistant microorganism, wherein (a) a stool sample of a mammal is incubated with at least two different monoclonal antibodies, fragments or derivatives thereof or aptamers under conditions allowing a complex formation of antigens of the acid-resistant microorganism with antibodies, fragments or derivatives thereof or the aptamers, and wherein (aa) the first monoclonal antibody or the fragment or the derivative thereof or the first aptamer specifically binds an epitope of the first antigen, which shows at least with some mammals a structure after the intestinal passage that corresponds to the native structure or the structure which a mammal produces antibodies against after being infected or immunised with the acid-resistant microorganism or an extract or lysate thereof or a protein therefrom or a fragment thereof or a synthetic peptide; (ab) the second monoclonal antibody or the fragment or the derivative thereof or the second aptamer specifically binds an epitope of a second antigen differing from the epitope of the first antigen, which shows at least with some mammals a structure after the intestinal passage that corresponds to the native structure or the structure which a mammal produces antibodies against after being infected or immunised with the acid-resistant microorganism or an extract or lysate thereof or a protein therefrom or a fragment thereof or a synthetic peptide, wherein the parts of the mammals may overlap according to (aa) and (ab) and in total essentially make up the overall number of infected mammals; and (b) the formation of at least one antigen-antibody complex or antigen-aptamer complex according to (aa) or (ab) is detected.
摘要翻译： 本发明涉及一种用耐酸微生物检测哺乳动物的感染的方法，其中（a）将哺乳动物的粪便样品与至少两种不同的单克隆抗体，其片段或衍生物或适体在允许 用抗体，其片段或衍生物或适配体复合形成抗酸微生物的抗原，并且其中（aa）第一单克隆抗体或其片段或其衍生物或第一适体特异性结合第一抗原的表位， 其至少与一些哺乳动物一起显示出肠道通过后的结构，该结构对应于哺乳动物产生针对抗酸微生物或其提取物或裂解物或蛋白质的免疫后的抗原的天然结构或结构，或 其片段或合成肽; （ab）第二单克隆抗体或其片段或其衍生物或第二适体特异性结合不同于第一抗原表位的第二抗原的表位，其至少显示出一些哺乳动物在肠道通过后对应的结构 哺乳动物产生抗体的抗原结构或结构，用抗酸微生物或其提取物或裂解物或其蛋白质或其片段或合成肽进行感染或免疫之后产生抗体，其中哺乳动物的部分可以 根据（aa）和（ab）重叠，总共基本上构成感染哺乳动物的总数; 和（b）检测根据（aa）或（ab）形成至少一种抗原 - 抗体复合物或抗原 - 适配子复合物。
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公开(公告)号：US20070009975A1
公开(公告)日：2007-01-11
申请号：US11513129
申请日：2006-08-31
申请人： Christian Reiter , Gerhard Cullman , Ulrike Friedrichs , Petra Heppner , Meret Lakner , Achim Ringeis
发明人： Christian Reiter , Gerhard Cullman , Ulrike Friedrichs , Petra Heppner , Meret Lakner , Achim Ringeis
IPC分类号： G01N33/554 , G01N33/569
CPC分类号： G01N33/56922 , C07K16/121 , G01N2333/205
摘要： The invention relates to a method for detecting an infection of a mammal with an acid-resistant microorganism, wherein (a) a stool sample of a mammal is incubated with at least two different monoclonal antibodies, fragments or derivatives thereof or aptamers under conditions allowing a complex formation of antigens of the acid-resistant microorganism with antibodies, fragments or derivatives thereof or the aptamers, and wherein (aa) the first monoclonal antibody or the fragment or the derivative thereof or the first aptamer specifically binds an epitope of the first antigen, which shows at least with some mammals a structure after the intestinal passage that corresponds to the native structure or the structure which a mammal produces antibodies against after being infected or immunised with the acid-resistant microorganism or an extract or lysate thereof or a protein therefrom or a fragment thereof or a synthetic peptide; (ab) the second monoclonal antibody or the fragment or the derivative thereof or the second aptamer specifically binds an epitope of a second antigen differing from the epitope of the first antigen, which shows at least with some mammals a structure after the intestinal passage that corresponds to the native structure or the structure which a mammal produces antibodies against after being infected or immunised with the acid-resistant microorganism or an extract or lysate thereof or a protein therefrom or a fragment thereof or a synthetic peptide, wherein the parts of the mammals may overlap according to (aa) and (ab) and in total essentially make up the overall number of infected mammals; and (b) the formation of at least one antigen-antibody complex or antigen-aptamer complex according to (aa) or (ab) is detected.
摘要翻译： 本发明涉及一种用耐酸微生物检测哺乳动物的感染的方法，其中（a）将哺乳动物的粪便样品与至少两种不同的单克隆抗体，其片段或衍生物或适体在允许 用抗体，其片段或衍生物或适配体复合形成抗酸微生物的抗原，并且其中（aa）第一单克隆抗体或其片段或其衍生物或第一适体特异性结合第一抗原的表位， 其至少与一些哺乳动物一起显示出肠道通过后的结构，该结构对应于哺乳动物产生针对抗酸微生物或其提取物或裂解物或蛋白质的免疫后的抗原的天然结构或结构，或 其片段或合成肽; （ab）第二单克隆抗体或其片段或其衍生物或第二适体特异性结合不同于第一抗原表位的第二抗原的表位，其至少显示出一些哺乳动物在肠道通过后对应的结构 哺乳动物产生抗体的抗原结构或结构，用抗酸微生物或其提取物或裂解物或其蛋白质或其片段或合成肽进行感染或免疫之后产生抗体，其中哺乳动物的部分可以 根据（aa）和（ab）重叠，总共基本上构成感染哺乳动物的总数; 和（b）检测根据（aa）或（ab）形成至少一种抗原 - 抗体复合物或抗原 - 适配子复合物。
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公开(公告)号：US20050042675A1
公开(公告)日：2005-02-24
申请号：US10362976
申请日：2001-08-31
申请人： Meret Lakner , Andreas True , Sonja Dehnert , Christian Reiter , Gerhard Cullmann , Petra Heppner , Eva Haindl , Andreas Finck , Helmut Benesch , Achim Ringeis
发明人： Meret Lakner , Andreas True , Sonja Dehnert , Christian Reiter , Gerhard Cullmann , Petra Heppner , Eva Haindl , Andreas Finck , Helmut Benesch , Achim Ringeis
摘要： The present invention relates to a new solution for the preparation of stool samples for diagnostic tests. The solution allows samples to be prepared simply before use in an immunological detection process and also provides a high level of sensitivity, specificity and reproducibility of the tests. The solution must contain at least one buffer substance, one detergent and one blocking reagent. The Invention also relates to a process for the analysis of a stool sample for diagnostic purposes using the solution according to the invention.
摘要翻译： 本发明涉及用于诊断测试的粪便样品的制备的新解决方案。 该溶液允许在免疫检测过程中简单地制备样品，并且还提供测试的高水平的灵敏度，特异性和可重复性。 该溶液必须含有至少一种缓冲物质，一种洗涤剂和一种封闭剂。 本发明还涉及使用根据本发明的溶液来诊断粪便样品的方法。
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