公开(公告)号：US07393950B2

公开(公告)日：2008-07-01

申请号：US10232923

申请日：2002-08-29

申请人： Chun Liang ,  Dao-rong Feng ,  Zhi-ling Yu

发明人： Chun Liang ,  Dao-rong Feng ,  Zhi-ling Yu

IPC分类号： C07H21/04 ,  A61K31/70

CPC分类号： C12N15/113 ,  A61K38/00 ,  C12N2310/11 ,  C12N2310/315

摘要： Antisense oligonucleotides that inhibit expression of human replication-initiation protein as well as methods of preventing or treating hyperproliferative conditions using said oligonucleotides are disclosed. One aspect provides an antisense oligonucleotides that inhibits the expression of human replication-initiation protein and has a sequence complementary to at least a portion of a target sequence encoding a human replication-initiation gene. By administering a therapeutically effective amount of antisense oligonucleotides or by contacting the hyperproliferating cells with an effective amount of antisense oligonucleotides, expression of replication-initiation protein is inhibited. Methods of screening and testing active antisense oligonucleotides for their ability to inhibit gene expression are also disclosed.

摘要翻译： 公开了抑制人复制起始蛋白表达的反义寡核苷酸以及使用所述寡核苷酸预防或治疗过度增殖病症的方法。 一方面提供了抑制人复制起始蛋白的表达的反义寡核苷酸，并且具有与编码人复制起始基因的靶序列的至少一部分互补的序列。 通过施用治疗有效量的反义寡核苷酸或通过使过度增殖细胞与有效量的反义寡核苷酸接触，抑制复制起始蛋白的表达。 还公开了筛选和测试活性反义寡核苷酸抑制基因表达能力的方法。

公开(公告)号：US08318922B2

公开(公告)日：2012-11-27

申请号：US12126862

申请日：2008-05-24

申请人： Chun Liang ,  Dao-rong Feng ,  Zhi-ling Yu

发明人： Chun Liang ,  Dao-rong Feng ,  Zhi-ling Yu

IPC分类号： C07H21/00 ,  C07H21/02 ,  C07H2/04

CPC分类号： C12N15/113 ,  A61K38/00 ,  C12N2310/11 ,  C12N2310/315

摘要： Antisense oligonucleotides that inhibit expression of human replication-initiation protein as well as methods of preventing or treating hyperproliferative conditions using said oligonucleotides are disclosed. One aspect provides an antisense oligonucleotide that inhibits the expression of human replication-initiation protein and has a sequence complementary to at least a portion of a target sequence encoding a human replication-initiation gene. By administering a therapeutically effective amount of an antisense oligonucleotide or by contacting the hyperproliferating cells with an effective amount of one or more antisense oligonucleotides, expression of replication-initiation protein is inhibited. Methods of screening and testing active antisense oligonucleotides for their ability to inhibit gene expression are also disclosed.

摘要翻译： 公开了抑制人复制起始蛋白表达的反义寡核苷酸以及使用所述寡核苷酸预防或治疗过度增殖病症的方法。 一方面提供了抑制人复制起始蛋白的表达的反义寡核苷酸，并且具有与编码人复制起始基因的靶序列的至少一部分互补的序列。 通过施用治疗有效量的反义寡核苷酸或通过使过度增殖细胞与有效量的一种或多种反义寡核苷酸接触，抑制复制起始蛋白的表达。 还公开了筛选和测试活性反义寡核苷酸抑制基因表达能力的方法。