公开(公告)号：US09040273B2

公开(公告)日：2015-05-26

申请号：US14140298

申请日：2013-12-24

Applicant: Chung Yuan Christian University

Inventor： Hsi-Ya Huang ,  Wan-Ling Liu ,  Chia-Her Lin ,  Sheng-Han Lo ,  Tadena Brenda Singco ,  Chun-Chuen Yang

IPC: C12N11/02 ,  C12N11/06

CPC classification number: C12N11/06

Abstract: A simple, highly efficient, and environmentally friendly method of immobilizing macromolecules, particularly the biomolecules, to a solid carrier and thus forming a bioreactor, including the following steps: (1) providing the biomolecule and a leading molecule, and linking the biomolecule and the leading molecule by microwave to form an adsorbent; (2) providing a porous support material, and mixing the porous support material and the adsorbent to form a mixture; (3) treating the mixture by a vortex process such that the adsorbent being adsorbed and immobilized on the porous support material to form a bioreactor for biological reactions and biocatalysis; and the bioreactor can be further used for constructing a biological reaction system via incorporating a buffer solution and a ligand.

Abstract translation: 一种将大分子特别是生物分子固定在固体载体上并由此形成生物反应器的简单，高效和环保的方法，包括以下步骤：（1）提供生物分子和主要分子，并将生物分子和 通过微波形成吸附剂; （2）提供多孔载体材料，并将多孔载体材料和吸附剂混合以形成混合物; （3）通过涡旋法处理混合物，使得吸附剂被吸附并固定在多孔载体材料上以形成用于生物反应和生物催化的生物反应器; 并且生物反应器可以通过引入缓冲溶液和配体进一步用于构建生物反应体系。