公开(公告)号：US20180312791A1

公开(公告)日：2018-11-01

申请号：US15832150

申请日：2017-12-05

申请人： Chung Yuan Christian University

发明人： Yu-Tzu Huang ,  Wen-Yaw Chung ,  Bo-Wei Wu ,  Jyun-Ting Lai ,  Jie-Ru Xu

IPC分类号： C12M1/00

摘要： A reaction device for a tiny organism includes a chamber, a first support plate, a second support plate and a separation plate. The tiny organism and a culture medium are added in the chamber. The first support plate is disposed on an opening of the chamber. The second support plate is disposed on one side opposite to the side on which the first support plate is disposed, and a third hole and a fourth hole separately correspond to a first hole and a second hole of the first support plate. A first end of the separation plate is located inside the chamber, the first end of the separation plate is spaced from the bottom of the chamber by a predetermined distance, a second end of the separation plate passes through the first support plate to connect to the second support plate.

公开(公告)号：US20240309465A1

公开(公告)日：2024-09-19

申请号：US18322595

申请日：2023-05-24

申请人： Chung Yuan Christian University

发明人： Yu-Tzu Huang ,  Jui-Chuang Wu ,  Ting-Cih Huang

IPC分类号： C12Q1/689 ,  C12Q1/6844 ,  G01N33/53 ,  G01N33/543

CPC分类号： C12Q1/689 ,  C12Q1/6844 ,  G01N33/5308 ,  G01N33/54388 ,  C12Q1/6804

摘要： The invention provides a detection method of ammonia oxidation functional gene in water treatment, including the following steps. For a sample to be detected, ammonia oxidation functional genes are amplified by PCR, and primers used for PCR are modified by attaching biotin and digoxigenin to forward and reverse 5′ ends respectively. Next, on the basis of immunochemistry, antibodies are used for biorecognition, and nano colloidal gold particles are used as chromogenic substances. The sample is placed on a sample pad of a lateral flow immunoassay test strip, and the sample flows from the sample pad to an absorbent pad. Color development on both the test and control lines when the sample flows through a nitrocellulose membrane indicates a positive result; color development solely on the control line indicates a negative result and no color development indicates an invalid test.