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1.发明授权公开(公告)号：US07842490B2
公开(公告)日：2010-11-30
申请号：US11720079
申请日：2005-11-07
CPC分类号： C12P19/12 , C12P7/10 , C12P19/14 , Y02E50/16 , Y02E50/343
摘要： The present invention relates to a process for liquefaction and saccharification of polysaccharide containing biomasses, having a relatively high dry matter content. The present invention combines enzymatic hydrolysis with a type of mixing relying on the principle of gravity ensuring that the biomasses are subjected to mechanical forces, primarily shear and tear forces. Furthermore, the present invention relates to the further utilization of such processed biomasses, e.g. for subsequent fermentation into bio-ethanol, bio-gas, specialty carbohydrates for food and fees as well as carbon feedstock for processing into plastics and chemicals.
摘要翻译： 本发明涉及具有相对高的干物质含量的含多糖的生物质的液化和糖化方法。 本发明根据重力原理将酶水解与一种混合相结合，确保生物质受到机械力，主要是剪切力和撕裂力。 此外，本发明涉及进一步利用这种经处理的生物质，例如， 用于随后发酵成生物乙醇，生物气体，特殊碳水化合物用于食品和费用以及用于加工成塑料和化学品的碳原料。
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2.发明授权公开(公告)号：US07598069B2
公开(公告)日：2009-10-06
申请号：US12102976
申请日：2008-04-15
CPC分类号： C12P19/12 , C12P7/10 , C12P19/14 , Y02E50/16 , Y02E50/343
摘要： Disclosed are processes for liquefaction and saccharification of polysacharide containing biomasses having high dry matter content (>20%) and preferably possessing large average particle size. The polysaccharide containing biomasses are subject to enzymatic hydrolysis in a mixer that utilizes “free fall” mixing. “Free fall” mixing provides mechanical degradation of the biomass during hydrolysis and, in the case of lignocellulosic biomass, promotes movement of cellulase enzymes along cellulose chains, which improves enzymatic hydrolysis at high dry matter.
摘要翻译： 公开了具有高干物质含量（> 20％）且优选具有大的平均粒度的含有多糖的生物质的液化和糖化的方法。 含有多糖的生物质在利用“自由落体”混合的混合器中进行酶水解。 “自由落体”混合在水解期间提供生物质的机械降解，并且在木质纤维素生物质的情况下促进纤维素酶沿着纤维素链的移动，这改善了在高干物质下的酶水解。
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3.发明申请公开(公告)号：US20080182323A1
公开(公告)日：2008-07-31
申请号：US12102976
申请日：2008-04-15
IPC分类号： C08B37/00
CPC分类号： C12P19/12 , C12P7/10 , C12P19/14 , Y02E50/16 , Y02E50/343
摘要： Disclosed are processes for liquefaction and saccharification of polysacharide containing biomasses having high dry matter content (>20%) and preferably possessing large average particle size. The polysaccharide containing biomasses are subject to enzymatic hydrolysis in a mixer that utilizes “free fall” mixing. “Free fall” mixing provides mechanical degradation of the biomass during hydrolysis and, in the case of lignocellulosic biomass, promotes movement of cellulase enzymes along cellulose chains, which improves enzymatic hydrolysis at high dry matter.
摘要翻译： 公开了具有高干物质含量（> 20％）且优选具有大的平均粒度的含有多糖的生物质的液化和糖化的方法。 含有多糖的生物质在利用“自由落体”混合的混合器中进行酶水解。 “自由落体”混合在水解期间提供生物质的机械降解，并且在木质纤维素生物质的情况下促进纤维素酶沿着纤维素链的移动，这改善了在高干物质下的酶水解。
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4.发明申请公开(公告)号：US20080138862A1
公开(公告)日：2008-06-12
申请号：US11720079
申请日：2005-11-07
IPC分类号： C12P19/00
CPC分类号： C12P19/12 , C12P7/10 , C12P19/14 , Y02E50/16 , Y02E50/343
摘要： The present invention relates to a process for liquefaction and saccharification of polysaccharide containing biomasses, having a relatively high dry matter content. The present invention combines enzymatic hydrolysis with a type of mixing relying on the principle of gravity ensuring that the biomasses are subjected to mechanical forces, primarily shear and tear forces. Furthermore, the present invention relates to the further utilisation of such processed biomasses, e.g. for subsequent fermentation into bio-ethanol, bio-gas, specialty carbohydrates for food and fees as well as carbon feedstock for processing into plastics and chemicals.
摘要翻译： 本发明涉及具有相对高的干物质含量的含多糖的生物质的液化和糖化方法。 本发明根据重力原理将酶水解与一种混合相结合，确保生物质受到机械力，主要是剪切力和撕裂力。 此外，本发明涉及进一步利用这种经处理的生物质，例如， 用于随后发酵成生物乙醇，生物气体，特殊碳水化合物用于食品和费用以及用于加工成塑料和化学品的碳原料。
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5.发明授权Methods for reducing enzyme consumption in second generation bioethanol fermentation in the prescence of lignin 有权第二代生物乙醇发酵中木质素测定中消耗酶消耗的方法公开(公告)号：US07972826B2
公开(公告)日：2011-07-05
申请号：US12863601
申请日：2009-02-02
申请人： Jan Larsen , Henning Jørgensen
发明人： Jan Larsen , Henning Jørgensen
摘要： Provided are methods of liquefaction, saccharification and fermentation of pre-treated lignocellulosic biomass in production of bioethanol. Addition of PEG or surfactant is effective to enhance cellulase hydrolysis yields at high dry matter (>20%) at surprisingly low concentrations compared with comparable results in low dry matter hydrolysis. This effect is most pronounced at comparatively low cellulase loading
摘要翻译： 提供在生物乙醇生产中预处理的木质纤维素生物质的液化，糖化和发酵的方法。 与低干物质水解中可比较的结果相比，添加PEG或表面活性剂有助于在高干物质（> 20％）下以令人惊讶的低浓度提高纤维素酶水解产率。 在相对较低的纤维素酶负载量<7 FPU（g DM）-1下，这种作用最显着。 在高干物质含量下，通过加入少量的PEG或表面活性剂可以减少纤维素酶消耗而不损失水解产率。
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6.发明申请Methods for reducing enzyme consumption in second generation bioethanol fermentation in the presence of lignin. 有权在木质素存在下减少第二代生物乙醇发酵中酶消耗的方法。公开(公告)号：US20100291650A1
公开(公告)日：2010-11-18
申请号：US12863601
申请日：2009-02-02
申请人： Jan Larsen , Henning Jørgensen
发明人： Jan Larsen , Henning Jørgensen
IPC分类号： C12P7/10
摘要： Provided are methods of liquefaction, saccharification and fermentation of pre-treated lignocellulosic biomass in production of bioethanol. Addition of PEG or surfactant is effective to enhance cellulase hydrolysis yields at high dry matter (>20%) at surprisingly low concentrations compared with comparable results in low dry matter hydrolysis. This effect is most pronounced at comparatively low cellulase loading
摘要翻译： 提供在生物乙醇生产中预处理的木质纤维素生物质的液化，糖化和发酵的方法。 与低干物质水解中可比较的结果相比，添加PEG或表面活性剂有助于在高干物质（> 20％）下以令人惊讶的低浓度提高纤维素酶水解产率。 在相对较低的纤维素酶负载量<7 FPU（g DM）-1下，这种作用最显着。 在高干物质含量下，通过加入少量的PEG或表面活性剂可以减少纤维素酶消耗而不损失水解产率。
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7.发明授权公开(公告)号：US10180203B2
公开(公告)日：2019-01-15
申请号：US15182372
申请日：2016-06-14
申请人： Jan Larsen , Chen-Shan Kung , Yuan-Jui Lu
发明人： Jan Larsen , Chen-Shan Kung , Yuan-Jui Lu
摘要： An underwater pipe and connector assembly includes an underwater pipe having a pipe body and an annular connecting end section, a connector fixed in the connecting end section, and at least one reinforcing layer having a first fixed cover section fused to and fixedly covering the connecting end section, and a second fixed cover section fused to and fixedly covering an outer circumferential surface of the pipe body immediately adjacent the connecting end section. An outer coil is embedded between the first fixed cover section and the connecting end section and between the second fixed cover section and the outer circumferential surface of the pipe body immediately adjacent the connecting end section.
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8.发明申请公开(公告)号：US20150037856A1
公开(公告)日：2015-02-05
申请号：US14350081
申请日：2011-10-06
申请人： Jan Larsen , Martin Dan Jeppesen
发明人： Jan Larsen , Martin Dan Jeppesen
IPC分类号： C12P7/14
摘要： Methods are provided for improved processing of lignocellulosic biomass. Hydrothermally pretreated lignocellulosic biomass is subject to separate hydrolysis and fermentation (SHF) or prehydrolysed and subject to simultaneous saccharification and fermentation (SSF) at high initial loadings of cellulase enzymes, at least 15 FPU/g DM. The cellulase enzymes are subsequently recycled and used in subsequent hydrolysis cycles along with a lower dose supplementation of fresh enzyme. Loss of enzyme activity between hydrolysis cycles is offset by improved overall process advantage.
摘要翻译： 提供了改进木质纤维素生物质处理的方法。 水热预处理的木质纤维素生物质经受单独的水解和发酵（SHF）或预水解，并在纤维素酶的高初始负载下进行同时糖化和发酵（SSF），至少15FPU / g DM。 随后将纤维素酶再循环使用，随后进行水解循环，同时补充新鲜的酶。 在水解循环之间的酶活性的损失被改进的整体工艺优点所抵消。
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公开(公告)号：US08187849B2
公开(公告)日：2012-05-29
申请号：US12808746
申请日：2008-12-18
申请人： Jan Larsen
发明人： Jan Larsen
CPC分类号： C12P7/06 , A01N43/08 , A01N61/00 , A01N65/00 , A01N65/44 , C12N1/22 , C12P7/10 , C12P2201/00 , Y02E50/16 , Y02E50/17
摘要： A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-bacterial treatment for first generation starch fermentations.
摘要翻译： 存在一定范围的浓度，其中衍生自木质纤维素原料的预处理的发酵抑制剂抑制乳酸菌的生长而不影响发酵酵母。 通过优化发酵抑制剂的含量落在此范围内，木质纤维素生物质的酵母发酵可以在非无菌条件下进行，乙醇产量与在无菌条件下达到的相似。 优化的抑制剂水平可以通过在预处理期间和之后控制木质纤维素生物质的水/生物量比来实现，例如通过用预定量的淡水或再循环的处理溶液洗涤先前预处理的木质纤维素生物质的纤维部分。 来自木质纤维素生物质预处理的液体馏分或处理溶液的粗提取物也可为第一代淀粉发酵提供有效的抗菌处理。
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公开(公告)号：US08187848B2
公开(公告)日：2012-05-29
申请号：US13132926
申请日：2009-12-17
IPC分类号： C12P7/06
CPC分类号： C12P19/14 , C12P7/10 , C12P2201/00 , Y02E50/16 , Y02E50/17
摘要： The invention relates to methods of processing ensiled biomass for production of bioethanol or other fermentation products and, in particular, to methods that do not require expensive pretreatment (e.g. heat or chemical pretreatment).
摘要翻译： 本发明涉及加工用于生产生物乙醇或其他发酵产物的生物质的方法，特别涉及不需要昂贵的预处理（例如热或化学预处理）的方法。
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