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公开(公告)号:US20190185843A1
公开(公告)日:2019-06-20
申请号:US16319746
申请日:2016-12-15
Applicant: DSM IP ASSETS B.V.
CPC classification number: C12N15/102 , C12N15/111 , C12N15/902 , C12N2310/20 , C12Q2521/301
Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct.The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.