公开(公告)号：US20190185843A1

公开(公告)日：2019-06-20

申请号：US16319746

申请日：2016-12-15

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries ROUBOS ,  René VERWAAL ,  Blance Elisabeth Maria GIELESEN ,  Brenda VONK

IPC: C12N15/10 ,  C12N15/11 ,  C12N15/90

CPC classification number: C12N15/102 ,  C12N15/111 ,  C12N15/902 ,  C12N2310/20 ,  C12Q2521/301

Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct.The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.