公开(公告)号：US11944104B2

公开(公告)日：2024-04-02

申请号：US17045062

申请日：2019-04-04

Applicant: DSM IP ASSETS B.V.

Inventor： Joke Anneleen Putseys ,  Aloysius Wilhelmus Rudolphus Hubertus Teunissen ,  René Marcel De Jong ,  Jeroen Godefrooij ,  Helma Arina Stolze-Lagerweij

IPC: C12N9/26 ,  A21D8/04

CPC classification number: A21D8/042 ,  C12N9/2414 ,  C12Y302/01133

Abstract: The present invention relates to a variant polypeptide having maltogenic alpha-amylase activity wherein the polypeptide comprises an amino acid sequence, which, when aligned with an amino acid sequence of SEQ ID NO: 1, comprises an amino acid substitution F188L/I, S200N, and D261G, and optionally a further amino acid substitution T288P, wherein the amino acid substitutions are determined with reference to SEQ ID NO: 1, and wherein the polypeptide has an amino acid sequence which has at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or at least 99% identity to the amino acid sequence of SEQ ID NO: 1. The invention also relates to a process for the preparation of a dough or a baked product wherein the variant polypeptide is used.