公开(公告)号：US20210115418A1

公开(公告)日：2021-04-22

申请号：US17253266

申请日：2019-06-18

Applicant: DSM IP ASSETS B.V.

Inventor： Joke Anneleen PUTSEYS ,  Neil CARR ,  René Marcel DE JONG ,  Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN

IPC: C12N9/20 ,  A21D8/04 ,  A21D6/00

Abstract: The present invention relates to a variant polypeptide having a lipase activity, wherein the polypeptide may be a polypeptide that has an amino acid sequence which has at least 70% identity to a mature amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 6, wherein the polypeptide comprises an amino acid substitution at position 295 and at least one further amino acid substitution at position 113, 121, 179 and/or 284, wherein the positions are defined with reference to SEQ ID NO: 1 or SEQ ID NO: 6. The invention further relates to a process for preparing a dough wherein a polypeptide as disclosed herein is used and baked product prepared from the dough.

公开(公告)号：US20210161158A1

公开(公告)日：2021-06-03

申请号：US17045062

申请日：2019-04-04

Applicant: DSM IP ASSETS B.V.

Inventor： Joke Anneleen PUTSEYS ,  Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN ,  René Marcel DE JONG ,  Jeroen GODEFROOIJ ,  Helma Arina STOLZE-LAGERWEIJ

IPC: A21D8/04 ,  C12N9/26

Abstract: The present invention relates to a variant polypeptide having maltogenic alpha-amylase activity wherein the polypeptide comprises an amino acid sequence, which, when aligned with an amino acid sequence of SEQ ID NO: 1, comprises an amino acid substitution F188L/I, S200N, and D261G, and optionally a further amino acid substitution T288P, wherein the amino acid substitutions are determined with reference to SEQ ID NO: 1, and wherein the polypeptide has an amino acid sequence which has at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or at least 99% identity to the amino acid sequence of SEQ ID NO: 1. The invention also relates to a process for the preparation of a dough or a baked product wherein the variant polypeptide is used.