公开(公告)号：US09951339B2

公开(公告)日：2018-04-24

申请号：US14427698

申请日：2013-09-19

Applicant: DSM IP ASSETS B.V.

Inventor： Ilse De Lange ,  Bernard Meijrink ,  Johannes Andries Roubos ,  Siew-loon Ooi

IPC: C12N15/65 ,  C12N15/80 ,  C12N15/81

CPC classification number: C12N15/65 ,  C12N15/80 ,  C12N15/81

Abstract: The invention relates to a method for modification of a host cell at a target locus, which method comprises: providing a host cell comprising, at a first locus, at least two site-specific recombination sites and a nucleic acid having an essential function or encoding a product having an essential function; introducing into the host cell, at the target locus, a further nucleic acid having the essential function or encoding for a product having the essential function; and carrying out recombination at the first locus via the at least two site-specific recombination sites, so that the nucleic acid having an essential function or encoding a product having an essential function is rendered non-functional, thereby to modify the host cell at the target locus. The invention also relates to a cell obtainable by a method of the invention.

公开(公告)号：US20150225728A1

公开(公告)日：2015-08-13

申请号：US14427698

申请日：2013-09-19

Applicant: DSM IP ASSETS B.V.

Inventor： Ilse De Lange ,  Bernard Meijrink ,  Johannes Andries Roubos ,  Siew-loon Ooi

IPC: C12N15/65 ,  C12N15/80

CPC classification number: C12N15/65 ,  C12N15/80 ,  C12N15/81

Abstract: The invention relates to a method for modification of a host cell at a target locus, which method comprises: providing a host cell comprising, at a first locus, at least two site-specific recombination sites and a nucleic acid having an essential function or encoding a product having an essential function; introducing into the host cell, at the target locus, a further nucleic acid having the essential function or encoding for a product having the essential function; and carrying out recombination at the first locus via the at least two site-specific recombination sites, so that the nucleic acid having an essential function or encoding a product having an essential function is rendered non-functional, thereby to modify the host cell at the target locus. The invention also relates to a cell obtainable by a method of the invention.

Abstract translation: 本发明涉及用于在靶标位点修饰宿主细胞的方法，该方法包括：提供宿主细胞，其在第一场所包含至少两个位点特异性重组位点和具有基本功能或编码的核酸 具有基本功能的产品; 在靶标位点向宿主细胞中引入具有基本功能或编码具有基本功能的产品的另一种核酸; 并通过至少两个位点特异性重组位点在第一位点进行重组，使得具有基本功能或编码具有基本功能的产物的核酸变得不起作用，从而修饰宿主细胞 目标位点。 本发明还涉及可通过本发明的方法获得的细胞。