-
公开(公告)号：US09127266B2
公开(公告)日：2015-09-08
申请号：US14075711
申请日：2013-11-08
Applicant: DSM IP ASSETS B.V.
Inventor： Jan Metske Van Der Laan , Mark Cristiaan Stor , Ilse De Lange , Lisette Mohrmann
CPC classification number: C12N9/82 , A23L5/25 , A23L29/06 , C12Y305/01001
Abstract: The present invention relates to newly identified asparaginase polypeptide variants of SEQ ID NO: 3 and to polynucleotide sequences that encode such novel asparaginase variants. Furthermore the invention relates to the use of these novel asparaginase variants in industrial processes.
Abstract translation: 本发明涉及新鉴定的SEQ ID NO：3的天冬酰胺酶多肽变体和编码这种新型天冬酰胺酶变体的多核苷酸序列。 此外，本发明涉及这些新型天冬酰胺酶变体在工业过程中的用途。
-
2.发明申请CELL MODIFICATION METHOD USING ESSENTIAL GENES AS MARKERS AND OPTIONALLY RECYCLING THESE 有权使用基因基因作为标记的细胞修饰方法和可循环的这些方法公开(公告)号：US20150225728A1
公开(公告)日：2015-08-13
申请号：US14427698
申请日：2013-09-19
Applicant: DSM IP ASSETS B.V.
Inventor： Ilse De Lange , Bernard Meijrink , Johannes Andries Roubos , Siew-loon Ooi
Abstract: The invention relates to a method for modification of a host cell at a target locus, which method comprises: providing a host cell comprising, at a first locus, at least two site-specific recombination sites and a nucleic acid having an essential function or encoding a product having an essential function; introducing into the host cell, at the target locus, a further nucleic acid having the essential function or encoding for a product having the essential function; and carrying out recombination at the first locus via the at least two site-specific recombination sites, so that the nucleic acid having an essential function or encoding a product having an essential function is rendered non-functional, thereby to modify the host cell at the target locus. The invention also relates to a cell obtainable by a method of the invention.
Abstract translation: 本发明涉及用于在靶标位点修饰宿主细胞的方法，该方法包括：提供宿主细胞，其在第一场所包含至少两个位点特异性重组位点和具有基本功能或编码的核酸 具有基本功能的产品; 在靶标位点向宿主细胞中引入具有基本功能或编码具有基本功能的产品的另一种核酸; 并通过至少两个位点特异性重组位点在第一位点进行重组，使得具有基本功能或编码具有基本功能的产物的核酸变得不起作用，从而修饰宿主细胞 目标位点。 本发明还涉及可通过本发明的方法获得的细胞。
-
公开(公告)号：US09951339B2
公开(公告)日：2018-04-24
申请号：US14427698
申请日：2013-09-19
Applicant: DSM IP ASSETS B.V.
Inventor： Ilse De Lange , Bernard Meijrink , Johannes Andries Roubos , Siew-loon Ooi
Abstract: The invention relates to a method for modification of a host cell at a target locus, which method comprises: providing a host cell comprising, at a first locus, at least two site-specific recombination sites and a nucleic acid having an essential function or encoding a product having an essential function; introducing into the host cell, at the target locus, a further nucleic acid having the essential function or encoding for a product having the essential function; and carrying out recombination at the first locus via the at least two site-specific recombination sites, so that the nucleic acid having an essential function or encoding a product having an essential function is rendered non-functional, thereby to modify the host cell at the target locus. The invention also relates to a cell obtainable by a method of the invention.
-
公开(公告)号：US09322045B2
公开(公告)日：2016-04-26
申请号：US14249587
申请日：2014-04-10
Applicant: DSM IP ASSETS B.V.
Inventor： Noël Nicolaas Maria Elisabeth Van Peij , Herman Jan Pel , Thibaut José Wenzel , Adriana Marina Riemens , Ilse De Lange
CPC classification number: C12P21/00 , A01K2267/01 , C12N9/2428 , C12N15/80 , C12N15/8509 , C12N15/902
Abstract: The present invention relates to a recombinant host cell for the production of a compound of interest. The invention further relates to a method for the production of such host cell. The invention further relates to the production of a compound of interest. The invention further relates to isolated polynucleotides and vectors and host cells comprising said polynucleotides.
Abstract translation: 本发明涉及用于生产感兴趣的化合物的重组宿主细胞。 本发明还涉及生产这种宿主细胞的方法。 本发明还涉及生产感兴趣的化合物。 本发明还涉及分离的多核苷酸和包含所述多核苷酸的载体和宿主细胞。
-
-
-
Search Results
4
records
(took 0.015 seconds)
