公开(公告)号：US20180251798A1

公开(公告)日：2018-09-06

申请号：US15970018

申请日：2018-05-03

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Adrianus Maria DE BONT ,  Aloysius TEUNISSEN ,  Paul KLAASSEN ,  Wouter Willem Antonius HARTMAN ,  Shimaira VAN BEUSEKOM

IPC: C12P7/06 ,  C12P7/10 ,  C12N1/22 ,  C12N9/04 ,  C12N9/02 ,  C12P7/08

CPC classification number: C12P7/06 ,  C12N1/22 ,  C12N9/0006 ,  C12N9/0008 ,  C12P7/08 ,  C12P7/10 ,  C12Y101/01006 ,  C12Y102/01 ,  C12Y102/0101 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising, hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD+-linked glycerol dehydrogenase activity. The process is further characterised in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell. The yeast cell further preferably comprises a functional exogenous xylose isomerase gene and/or functional exogenous genes which confer to the cell the ability to convert L-arabinose into D-xylulose 5-phosphate and they may comprise a genetic modification that increase acetyl-CoA synthetase activity.

公开(公告)号：US20190330664A1

公开(公告)日：2019-10-31

申请号：US16509700

申请日：2019-07-12

Applicant: DSM IP ASSETS B.V.

Inventor： Paul KLAASSEN ,  Wouter Willem Antonius HARTMAN

IPC: C12P7/10 ,  C12N1/16 ,  C12N9/02 ,  C12N9/12 ,  C12P7/06 ,  C12N9/04 ,  C12N9/00

Abstract: Cell that is genetically modified comprising: a) one or more nucleotide sequence encoding a NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10); b) one or more nucleotide sequence encoding a acetyl-CoA synthetase (E.C. 6.2.1.1); c) one or more nucleotide sequence encoding a glycerol dehydrogenase (E.C. 1.1.1.6); and d) one or more nucleotide sequence encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 or E.C. 2.7.1.29).