公开(公告)号：US20220145211A1

公开(公告)日：2022-05-12

申请号：US17439134

申请日：2020-03-13

Applicant: DSM IP Assets B.V.

Inventor： Negash ADUGNA

IPC: C11B1/02 ,  C11B3/00 ,  C11B3/16 ,  C07C51/42 ,  C07C51/48

Abstract: The present invention is directed to a process for obtaining a lipid from a composition comprising microbial cells, the process comprising: treating the composition comprising microbial cells to a temperature of about 60° C. to about 95° C. at a pH of about 9 to about 11; separating the treated microbial cells into a light phase and a heavy phase; sequentially, in one or more cycles, raising the pH of the concentrated microbial cell composition to 10 or above, followed by lowering the pH of the concentrated microbial cell composition to a pH of less than 6 to lyse the concentrated microbial cell composition, wherein the raising and lowering of the pH also demulsifies the concentrated microbial cell composition; separating the lipid from the demulsified cell composition, and recovering the lipid.

公开(公告)号：US20210017467A1

公开(公告)日：2021-01-21

申请号：US17042791

申请日：2019-03-29

Applicant: DSM IP Assets B.V. ,  Evonik Operations GmbH

Inventor： Negash ADUGNA ,  Martin HEINING ,  Michael Benjamin JOHNSON ,  Neil Francis LEININGER ,  Kirt Lyvell MATTHEWS, Sr. ,  Ginger Marie SHANK ,  Nasrin TABAYEHNEJAD ,  Vinod TARWADE

IPC: C11B1/02 ,  C11B1/10

Abstract: Disclosed herein are processes for reducing or eliminating emulsion during the process of obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by washing the fermentation broth with at least 1× amount of water. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

公开(公告)号：US20220154098A1

公开(公告)日：2022-05-19

申请号：US17439154

申请日：2020-03-13

Applicant: DSM IP Assets B.V.

Inventor： Negash ADUGNA

IPC: C11B1/02 ,  C11B3/16 ,  C11B3/04 ,  C11B3/06

Abstract: The present invention is directed to a process for obtaining a lipid from a cell, the process involving lysing a cell to form a lysed cell composition; optionally adding a salt to the lysed cell composition; sequentially, in one or more cycles, raising the pH of the lysed cell composition to 10 or above for a period of time, followed by lowering the pH of the lysed cell composition to a pH of less than 6 for a period of time to demulsify the cell composition; and separating a lipid from the demulsified cell composition.