公开(公告)号：US20250102505A1

公开(公告)日：2025-03-27

申请号：US18728404

申请日：2021-05-26

Applicant: DYNAMIC BIOSENSORS GMBH

Inventor： Nena MATSCHEKO ,  Hanna MÜLLER-LANDAU ,  Ulrich RANT ,  Sina REEDE ,  Michael VELLEKOOP ,  Frieder LUCKLUM

IPC: G01N33/569 ,  B01L3/00 ,  G01N33/543

Abstract: The present invention relates to methods for the individual immobilization of one or more cells under fluid flow conditions, in the context of a bioassay, comprising for each cell the step of trapping said cell in a reticular structure that is arranged in a microfluidic channel of a microfluidic device in a direction that is perpendicular to the direction of fluid flow. Further, the present invention relates to respective bioassays comprising the step of individually immobilizing one or more cells under fluid flow conditions according to said methods, e.g. assays for determining the kinetics of the binding of a ligand to the immobilized cells. Furthermore, the present invention relates to respective microfluidic devices.