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    CELL IMMOBILIZATION IN FLUID AT HIGH FLOW VELOCITIES
    1.
    发明申请
    CELL IMMOBILIZATION IN FLUID AT HIGH FLOW VELOCITIES 有权

    公开(公告)号：US20250102505A1

    公开(公告)日：2025-03-27

    申请号：US18728404

    申请日：2021-05-26

    Applicant: DYNAMIC BIOSENSORS GMBH

    Inventor： Nena MATSCHEKO ,  Hanna MÜLLER-LANDAU ,  Ulrich RANT ,  Sina REEDE ,  Michael VELLEKOOP ,  Frieder LUCKLUM

    IPC: G01N33/569 ,  B01L3/00 ,  G01N33/543

    Abstract: The present invention relates to methods for the individual immobilization of one or more cells under fluid flow conditions, in the context of a bioassay, comprising for each cell the step of trapping said cell in a reticular structure that is arranged in a microfluidic channel of a microfluidic device in a direction that is perpendicular to the direction of fluid flow. Further, the present invention relates to respective bioassays comprising the step of individually immobilizing one or more cells under fluid flow conditions according to said methods, e.g. assays for determining the kinetics of the binding of a ligand to the immobilized cells. Furthermore, the present invention relates to respective microfluidic devices.

    METHOD FOR SEQUENCING A TEMPLATE NUCLEIC ACID IMMOBILIZED ON A SUBSTRATE
    7.
    发明申请
    METHOD FOR SEQUENCING A TEMPLATE NUCLEIC ACID IMMOBILIZED ON A SUBSTRATE 审中-公开
    用于测定在基底上固定的模板核酸的方法

    公开(公告)号：US20150368705A1

    公开(公告)日：2015-12-24

    申请号：US14763078

    申请日：2014-01-22

    Applicant: DYNAMIC BIOSENSORS GMBH

    Inventor： Andreas LANGER ,  Ralf STRASSER ,  Ulrich RANT

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q2565/607

    Abstract: The present invention is directed to sequencing of nucleic acids. A method is provided for sequencing based on immobilized nucleic acid on a surface. Advantageously, a long range detection mechanism is used for detecting, whether a nucleotide provided to the substrate of a biochip has been incorporated into the immobilized template nucleic acid. Various different alignment means are provided by the present invention which can be used for facilitating a rigidly locking of the orientation of the DNA complex, which complex comprises the template nucleic acid, the primer and the capture nucleic acid. Various different linker systems may be used to immobilize the DNA complex at a first and a second strand end, such that the desired alignment of the DNA complex is achieved. Also co-adsorbed molecules on the substrate surface can be used for such an aligning measure. Additionally, or alternatively, an electrical field may be applied for repelling the DNA complex from the electrode and for facilitating a vertical DNA complex orientation. Advantageously, label-free nucleotides can be used, if desired.

    Abstract translation: 本发明涉及核酸的测序。 提供了一种基于表面上固定化核酸进行测序的方法。 有利地，远程检测机制用于检测提供给生物芯片的底物的核苷酸是否已经并入固定的模板核酸中。 通过本发明提供了各种不同的对准装置，其可用于促进DNA复合物的取向的刚性锁定，该复合物包含模板核酸，引物和捕获核酸。 可以使用各种不同的接头体系来固定DNA复合物在第一和第二链末端，从而实现DNA复合物的所需比对。 也可以将基底表面上的共吸附分子用于这种对准测量。 另外或替代地，可以施加电场以从电极排斥DNA复合物并促进垂直DNA复合物取向。 有利地，如果需要，可以使用无标记的核苷酸。

    Method for sequencing a template nucleic acid immobilized on a substrate
    8.
    发明授权
    Method for sequencing a template nucleic acid immobilized on a substrate 有权

    公开(公告)号：US10081834B2

    公开(公告)日：2018-09-25

    申请号：US14763078

    申请日：2014-01-22

    Applicant: DYNAMIC BIOSENSORS GMBH

    Inventor： Andreas Langer ,  Ralf Strasser ,  Ulrich Rant

    IPC: C12Q1/68 ,  C12Q1/6869 ,  C07H21/00 ,  C12Q1/6825 ,  C12Q1/6834

    CPC classification number: C12Q1/6869 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q2565/607

    Abstract: The present invention is directed to sequencing of nucleic acids. A method is provided for sequencing based on immobilized nucleic acid on a surface. Advantageously, a long range detection mechanism is used for detecting, whether a nucleotide provided to the substrate of a biochip has been incorporated into the immobilized template nucleic acid. Various different alignment means are provided by the present invention which can be used for facilitating a rigidly locking of the orientation of the DNA complex, which complex comprises the template nucleic acid, the primer and the capture nucleic acid. Various different linker systems may be used to immobilize the DNA complex at a first and a second strand end, such that the desired alignment of the DNA complex is achieved. Also co-adsorbed molecules on the substrate surface can be used for such an aligning measure. Additionally, or alternatively, an electrical field may be applied for repelling the DNA complex from the electrode and for facilitating a vertical DNA complex orientation. Advantageously, label-free nucleotides can be used, if desired.

    Apparatus and Method for Evaluating Characteristics of Target Molecules
    10.
    发明申请
    Apparatus and Method for Evaluating Characteristics of Target Molecules 有权
    用于评估目标分子特征的装置和方法

    公开(公告)号：US20160011148A1

    公开(公告)日：2016-01-14

    申请号：US14856852

    申请日：2015-09-17

    Applicant: Dynamic Biosensors GmbH

    Inventor： Ulrich Rant ,  Wolfgang Kaiser ,  Paul Andreas Hampel ,  Jens Niemax ,  Andreas Langer ,  Jelena Knezevic

    IPC: G01N27/447

    CPC classification number: G01N27/44726 ,  C12Q1/6823 ,  C12Q1/6825 ,  G01N21/6408 ,  G01N21/6458 ,  G01N27/327 ,  G01N27/44756 ,  G01N33/5438 ,  G01N2021/6432 ,  C12Q2537/165 ,  C12Q2561/12 ,  C12Q2563/116

    Abstract: Arrangements are described for evaluating characteristics of target molecules. A biochip is received which includes a substrate to which charged probe molecules are attached. The probe molecules have a marker to allow generating signals indicative of the distance of a portion of the probe molecule from the substrate. The signals are detected and means for an external electric field is generated to which the probe molecules are exposed. A control means acts to: (A) apply an external electric field causing the portion of the probe molecule to approach the substrate, and (B) apply an external electric field causing the portion of the probe molecule to move away from the substrate. The signal is recorded as a function of time during step (A) and/or step (B). Steps (A) and (B) are repeated for a predetermined number of times and the recorded signals are combined.

    Abstract translation: 描述了评估靶分子特征的安排。 接收生物芯片，其包括附着有带电探针分子的基底。 探针分子具有标记以允许产生指示探针分子的一部分与基底的距离的信号。 检测信号，产生探针分子所暴露的外部电场的装置。 控制装置用于：（A）施加外部电场，使得探针分子的该部分接近衬底，和（B）施加外部电场，使得探针分子的该部分离开衬底。 在步骤（A）和/或步骤（B）期间，该信号被记录为时间的函数。 重复步骤（A）和（B）预定次数并组合记录信号。

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