公开(公告)号：US20130304397A1

公开(公告)日：2013-11-14

申请号：US13882641

申请日：2011-10-31

申请人： Dag Erlandsson ,  Martin Hedstrom ,  Bo Mattiasson ,  Joakim Larsson

发明人： Dag Erlandsson ,  Martin Hedstrom ,  Bo Mattiasson ,  Joakim Larsson

IPC分类号： G01N27/22 ,  G01R27/26

CPC分类号： G01N27/22 ,  G01N27/228 ,  G01N27/3276 ,  G01R27/26 ,  G01R27/2605

摘要： A method of measuring a capacitance (C) of a sensor having a working electrode that is coated by an insulating layer and a ligand forming an affinity surface. The method involves the steps of bringing the electrode into contact with an analyte, supplying a constant first current (I1), and a constant second current (I2) of opposite direction to the first current (I1), and a constant third current (I3) of same direction as the first current (I1) during determined time periods to the sensor. Further the method includes sampling the potential (V) built up across the sensor, and calculating the capacitance of the sensor from the inclination (B, D, F, H) of a potential curve received in response to the current supply. A method used for detection of an interaction between a ligand and an analyte is also disclosed.

摘要翻译： 一种测量具有由绝缘层涂覆的工作电极和形成亲和表面的配体的传感器的电容（C）的方法。 该方法包括使电极与分析物接触的步骤，提供与第一电流（I1）相反方向的恒定的第一电流（I1）和恒定的第二电流（I2），以及恒定的第三电流（I3 ）与在传感器确定的时间段期间的与第一电流（I1）相同的方向。 此外，该方法包括对跨传感器建立的电位（V）进行采样，以及响应于当前电源接收的电位曲线的倾斜度（B，D，F，H）计算传感器的电容。 还公开了用于检测配体和分析物之间相互作用的方法。

公开(公告)号：US09304096B2

公开(公告)日：2016-04-05

申请号：US13882641

申请日：2011-10-31

申请人： Dag Erlandsson ,  Martin Hedstrom ,  Bo Mattiasson ,  Joakim Larsson

发明人： Dag Erlandsson ,  Martin Hedstrom ,  Bo Mattiasson ,  Joakim Larsson

IPC分类号： G01R29/26 ,  G01N27/22 ,  G01N27/327 ,  G01R27/26

CPC分类号： G01N27/22 ,  G01N27/228 ,  G01N27/3276 ,  G01R27/26 ,  G01R27/2605

摘要： A method of measuring a capacitance (C) of a sensor having a working electrode that is coated by an insulating layer and a ligand forming an affinity surface. The method involves the steps of bringing the electrode into contact with an analyte, supplying a constant first current (I1), and a constant second current (I2) of opposite direction to the first current (I1), and a constant third current (I3) of same direction as the first current (I1) during determined time periods to the sensor. Further the method includes sampling the potential (V) built up across the sensor, and calculating the capacitance of the sensor from the inclination (B, D, F, H) of a potential curve received in response to the current supply. A method used for detection of an interaction between a ligand and an analyte is also disclosed.

摘要翻译： 一种测量具有由绝缘层涂覆的工作电极和形成亲和表面的配体的传感器的电容（C）的方法。 该方法包括使电极与分析物接触的步骤，提供与第一电流（I1）相反方向的恒定的第一电流（I1）和恒定的第二电流（I2），以及恒定的第三电流（I3 ）与在传感器确定的时间段期间的与第一电流（I1）相同的方向。 此外，该方法包括对跨传感器建立的电位（V）进行采样，以及响应于当前电源接收的电位曲线的倾斜度（B，D，F，H）计算传感器的电容。 还公开了用于检测配体和分析物之间相互作用的方法。