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公开(公告)号：US06461834B1
公开(公告)日：2002-10-08
申请号：US09212663
申请日：1998-12-16
IPC分类号： C12P2106
CPC分类号： C07K14/605 , C12P21/06
摘要： The invention provides a method of producing a polypeptide having a C-terminal &agr;-carboxamide group. It particularly concerns an enzymatic modification of selected substrate polypeptides which result in cleavage of the substrate polypeptide to form a product peptide with a C-terminal arginine residue having an &agr;-carboxamide group (C-terminal “Arg-NH2”). The method includes contacting an aqueous-based solution including (i) ammonia reagent and (ii) the substrate polypeptide with (iii) clostripain.
摘要翻译： 本发明提供了产生具有C-末端α-甲酰胺基团的多肽的方法。 它特别涉及所选择的底物多肽的酶修饰，其导致底物多肽切割以形成具有α-甲酰胺基团（C-末端“Arg-NH 2”）的C-末端精氨酸残基的产物肽。 该方法包括使含有（i）氨试剂和（ii）底物多肽的水性溶液与（iii）clostripain接触。
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2.发明授权Composition for palladium-mediated cleavage of peptides containing CXC, CXH or CHM sequences 有权用于钯介导的切割含有CXC，CXH或CHM序列的肽的组合物公开(公告)号：US08153762B2
公开(公告)日：2012-04-10
申请号：US12199902
申请日：2008-08-28
IPC分类号： C07K1/107
CPC分类号： C07K1/126 , C07K1/003 , C07K5/1008 , C07K5/1016 , C07K5/1021 , C07K7/06
摘要： The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxyl-terminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.
摘要翻译： 本发明提供了一种酰化所需肽的方法，其包括在X1-半胱氨酸序列处切割底物多肽，其中X1是肽羧基末端的氨基酸，半胱氨酸是包含序列半胱氨酸的钯切割位点的第一个氨基酸 -X2-X3，其中X2是任何氨基酸，X3是选自半胱氨酸，组氨酸或甲硫氨酸的氨基酸，并且其中在X1-半胱氨酸序列切割时肽的羧基末端酰胺化。
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