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    Process for performing retro-aldol reaction
    3.
    发明授权
    Process for performing retro-aldol reaction 失效
    进行醛缩醇反应的方法

    公开(公告)号：US5677423A

    公开(公告)日：1997-10-14

    申请号：US763584

    申请日：1996-12-10

    申请人： Michael J. Rodriguez

    发明人： Michael J. Rodriguez

    IPC分类号： C07C45/51 ,  C07C49/20 ,  C07K1/113 ,  C07K1/12 ,  C07K7/56 ,  C07K7/64 ,  C07K1/107 ,  C07C67/47

    CPC分类号： C07K7/64 ,  C07C45/512 ,  C07K1/126 ,  C07K7/56

    摘要： A process for removing .beta.-hydroxy groups from .beta.-hydroxy-containing compounds is disclosed. The process involves the use of a retro-aldol-promoting reagent selected from the group of trimethylamine-N-oxide, triethylamine-N-oxide, trimethylamine-N-oxide-hydrate, and trimethylamine-hydrate and requires dissolution of the substrate in an aprotic solvent and reaction under elevated temperatures. The process is broadly applicable to a variety of substrates including complex cyclic peptides, linear peptides, and non-peptides.

    摘要翻译： 公开了从含β-羟基的化合物中除去β-羟基的方法。 该方法包括使用选自三甲胺-N-氧化物，三乙胺-N-氧化物，三甲胺-N-氧化物 - 水合物和三甲胺水合物的反醛醇醇促进剂，并且需要将基质溶解在 非质子溶剂和在升高的温度下反应。 该方法广泛适用于各种底物，包括复合环肽，线性肽和非肽。

    Liquid phase peptide syntheses of KL-4 pulmonary surfactant
    4.
    发明申请
    Liquid phase peptide syntheses of KL-4 pulmonary surfactant 审中-公开
    KL-4肺表面活性剂的液相肽合成

    公开(公告)号：US20070149765A1

    公开(公告)日：2007-06-28

    申请号：US11581925

    申请日：2006-10-17

    申请人： Ahmed Abdel-Magid

    发明人： Ahmed Abdel-Magid

    IPC分类号： C07K7/08

    CPC分类号： C07K5/0808 ,  A61K38/00 ,  C07K1/126 ,  C07K5/0815 ,  C07K14/785

    摘要： The invention realtes to improved liquid phase processes for the preparation of the 21 residue protein component, (Lys-Leu4)4-Lys, of the pulmonary surfactant KL-4. These process are amenable to large scale synthesis and one process employs a method of saponifying an ester which reduces the inherent racemization of the α-carbon.

    摘要翻译： 本发明涉及用于制备肺表面活性剂KL-4的21残基蛋白组分（Lys-Leu 4 N 4）4 -Sys的改进的液相方法。 这些方法适合于大规模合成，一种方法采用皂化方法来降低α-碳的固有外消旋化。

    Peptide amidation process
    5.
    发明申请
    Peptide amidation process 有权
    肽酰胺化过程

    公开(公告)号：US20050287632A1

    公开(公告)日：2005-12-29

    申请号：US10997081

    申请日：2004-11-24

    申请人： Barton Holmquist ,  Daniel Strydom

    发明人： Barton Holmquist ,  Daniel Strydom

    IPC分类号： C12N15/09 ,  C07K1/00 ,  C07K1/107 ,  C07K5/103 ,  C07K5/107 ,  C07K5/113 ,  C07K7/06 ,  C07K7/08 ,  C07K14/575 ,  C07K14/60 ,  C12N1/21 ,  C12P21/06

    CPC分类号： C07K1/126 ,  C07K1/003 ,  C07K5/1008 ,  C07K5/1016 ,  C07K5/1021 ,  C07K7/06

    摘要： The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxylterminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.

    摘要翻译： 本发明提供了一种酰胺化所需肽的方法，其包括在X 1 - 半胱氨酸序列上切割底物多肽，其中X1是肽羧基末端的氨基酸，半胱氨酸是第一个氨基酸 包含序列半胱氨酸-X 2 -X 3的钯裂解位点，其中X 2是任何氨基酸，X 3是选自半胱氨酸，组氨酸或甲硫氨酸的氨基酸，并且其中所述肽的羧基末端在裂解时被酰胺化 X1半胱氨酸序列。

    Low-molecular weight peptide mixture and method of producing same
    10.
    发明授权
    Low-molecular weight peptide mixture and method of producing same 失效
    低分子量肽混合物及其制备方法

    公开(公告)号：US4940662A

    公开(公告)日：1990-07-10

    申请号：US794322

    申请日：1985-11-01

    申请人： Ken-ichi Yamazaki ,  Shoji Takao ,  Koji Yamamoto

    发明人： Ken-ichi Yamazaki ,  Shoji Takao ,  Koji Yamamoto

    IPC分类号： A23J3/00 ,  A23J3/34 ,  A61K38/00 ,  C07K1/12 ,  C12P21/02 ,  C12P21/06

    CPC分类号： C07K1/126 ,  C12P21/02 ,  C12P21/06

    摘要： A method of producing a low-molecular weight peptide mixture, comprising the steps of dissolving a first protease in a buffer solution adjusted to an optimum pH for the protease ranging from pH 3 to 10, adding at least one first protein in a buffer solution having a pH of from 3 to 10 and a concentration of 10 to 60% by weight of the protein material and thoroughly mixing the solution, adding a solution of an ester of at least one amino acid pre-formed by esterification of the amino acid with an alcohol, the pH of said buffer solution being optimum for the incorporation of said amino acid in said starting protein in the presence of said first protease in a plastein reaction, reacting said ester of at least one amino acid with said starting protein in a plastein reaction in the mixed solution whereby said at least one amino acid is covalently incorporated into said starting protein to produce a plastein reaction solution containing a modified protein, hydrolyzing said modified protein using at least one second protease having a different specificity from said at least one first protease to produce a low-molecular weight peptide mixture having an amino acid content which has different proportions of amino acids than does said starting protein, and separating from the solution said low-molecular weight peptide mixture which comprises a major proportion of dipeptides and tripeptides and containing not more than 15% by weight of free amino acids and not more than 20% by weight of a high-molecular weight fraction of compounds having a molecular weight of not lower than 700 and removable by gel filtration. The said low-molecular weight peptide mixture is readily absorbable and a useful nutrient.

    摘要翻译： 一种生产低分子量肽混合物的方法，包括以下步骤：将第一蛋白酶溶解在缓冲溶液中，调节至最适pH为pH3至10的蛋白酶，加入至少一种第一蛋白质的缓冲溶液中， pH为3至10，浓度为蛋白质材料的10至60重量％，并将溶液充分混合，加入通过氨基酸酯化预先形成的至少一个氨基酸的酯溶液与 醇，所述缓冲溶液的pH最适于在所述起始蛋白质中在所述第一蛋白酶存在下在Plastein反应中引入所述氨基酸，使所述至少一个氨基酸的所述酯与所述起始蛋白质在Plastein反应中反应 在所述混合溶液中，其中所述至少一个氨基酸共价掺入所述起始蛋白质中以产生含有修饰蛋白质的粘多糖反应溶液，水解所述修饰的蛋白质u 与至少一种第一蛋白酶具有不同特异性的至少一种第二蛋白酶，以产生具有与所述起始蛋白质不同比例的氨基酸的氨基酸含量的低分子量肽混合物，并从所述溶液中分离出所述第 低分子量肽混合物，其包含主要比例的二肽和三肽，并且含有不超过15重量％的游离氨基酸和不超过20重量％的高分子量级分的分子量为 不低于700，可通过凝胶过滤去除。 所述低分子量肽混合物易于吸收和有用的营养物质。