摘要:
A salicylic acid-induced protein (SIP) kinase is disclosed. The kinase has a molecular weight of about 48 kDa and is activated in response to salicylic acid, H.sub.2 O.sub.2 and infection with tobacco mosaic virus. The activation and enzymatic properties of the purified protein have been characterized. The partial amino acid sequence and complete nucleotide sequence of a cDNA encoding the SIP kinase demonstrate that it is a unique member of the mitogen-activated protein (MAP) kinase family. The novel SIP kinase may play a critical role in signal transduction for activation of plant defenses against microbial pathogens.
摘要翻译:公开了A + E,uns s + EE脂酰酸+ E,uns i + EE诱导的+ E,uns p + EE蛋白(SIP)激酶。 该激酶具有约48kDa的分子量,并响应于水杨酸,H 2 O 2和感染烟草花叶病毒而被激活。 已经表征了纯化的蛋白质的活化和酶学性质。 编码SIP激酶的cDNA的部分氨基酸序列和完整的核苷酸序列表明,它是+ E,uns m + EE itogen-+ E,uns a + EE激活+ E,uns p + EE蛋白的独特成员 (MAP)激酶家族。 新型SIP激酶可能在信号转导中起关键作用,用于激活植物防御微生物病原体。
摘要:
Novel used for WIPK, a member of the mitogen-activated protein (MAP) kinase family, are provided, based on the discovery that the WIPK protein is activatable in association with development or enhancement of resistance to microbial pathogens. Thus, WIPK may play a critical role in signal transduction for activation of plant defenses against certain microbial pathogens. Methods are disclosed for making WIPK transgenic plants with enchanced resistance to disease causing agents. In addition, transgenic plants transformed with WIPK and having enhanced disease resistance are disclosed.