公开(公告)号：US20140331360A1

公开(公告)日：2014-11-06

申请号：US14341999

申请日：2014-07-28

申请人： Danziger Innovations Ltd. ,  Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.

发明人： Alexander VAINSTEIN ,  Amir Zuker

IPC分类号： C12N15/82

CPC分类号： C12N15/8203 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8289

摘要： A method of generating genotypic variation in a genome of a plant is disclosed. The method comprising introducing into the plant at least one viral expression vector encoding at least one chimeric nuclease which comprises a DNA binding domain, a nuclease and a localization signal to a DNA-containing organelle, wherein the DNA binding domain mediates specific targeting of the nuclease to the genome of the plant, thereby generating genotypic variation in the genome of the plant.

摘要翻译： 公开了在植物基因组中产生基因型变异的方法。 该方法包括将至少一种编码至少一种嵌合核酸酶的病毒表达载体引入植物中，所述嵌合核酸酶包含DNA结合结构域，核酸酶和对含DNA细胞器的定位信号，其中所述DNA结合结构域介导核酸酶的特异性靶向 到植物的基因组，从而在植物的基因组中产生基因型变异。