公开(公告)号：US20090012280A1

公开(公告)日：2009-01-08

申请号：US12109856

申请日：2008-04-25

申请人： David A. Stein ,  Richard K. Bestwick ,  Patrick L. Iversen ,  Benjamin Neuman ,  Michael Buchmejer ,  Dwight D. Weller

发明人： David A. Stein ,  Richard K. Bestwick ,  Patrick L. Iversen ,  Benjamin Neuman ,  Michael Buchmejer ,  Dwight D. Weller

IPC分类号： C07H21/02

CPC分类号： C07H21/02 ,  C07K14/005 ,  C12N15/1131 ,  C12N2310/11 ,  C12N2310/3233 ,  C12N2310/3513 ,  C12N2770/20022

摘要： A method and oligonucleotide compound for inhibiting replication of a nidovirus in virus-infected animal cells are disclosed. The compound (i) has a nuclease-resistant backbone, (ii) is capable of uptake by the infected cells, (iii) contains between 8-25 nucleotide bases, and (iv) has a sequence capable of disrupting base pairing between the transcriptional regulatory sequences in the 5′ leader region of the positive-strand viral genome and negative-strand 3′ subgenomic region. In practicing the method, infected cells are exposed to the compound in an amount effective to inhibit viral replication.

摘要翻译： 公开了用于抑制病毒感染的动物细胞中的Nidovirus复制的方法和寡核苷酸化合物。 化合物（i）具有核酸酶抗性主链，（ii）能够被感染细胞摄取，（iii）含有8-25个核苷酸碱基之间，和（iv）具有能够破坏转录之间碱基配对的序列 正链病毒基因组和负链3'亚基因组区域的5'前导区域中的调节序列。 在实施该方法中，感染的细胞以有效抑制病毒复制的量暴露于化合物。