公开(公告)号：US12104151B2

公开(公告)日：2024-10-01

申请号：US18185048

申请日：2023-03-16

申请人： President and Fellows of Harvard College

发明人： Xiaowei Zhuang ,  Kok-Hao Chen ,  Alistair Boettiger ,  Jeffrey R. Moffitt ,  Siyuan Wang

IPC分类号： C12N15/10 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6869 ,  G16B25/00 ,  G16B25/20 ,  G16B30/00 ,  G16B40/00 ,  G16B40/10 ,  G16C20/10 ,  G06N7/01

CPC分类号： C12N15/1065 ,  C07H21/02 ,  C07H21/04 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6837 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10 ,  G16C20/10 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6869 ,  G06N7/01 ,  G16B30/00 ,  G16B40/00 ,  C12Q1/6806 ,  C12Q2521/107 ,  C12Q2525/143 ,  C12Q2525/179 ,  C12Q2537/143 ,  C12Q2565/514 ,  C12Q1/6816 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q1/6816 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q2565/102

摘要： The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

公开(公告)号：US12103944B2

公开(公告)日：2024-10-01

申请号：US18408431

申请日：2024-01-09

申请人： TriLink BioTechnologies, LLC

发明人： Richard I. Hogrefe ,  Alexandre Lebedev ,  Anton P. McCaffrey ,  Dongwon Shin

IPC分类号： C07H21/02 ,  A23L33/13 ,  A61K31/711 ,  A61K31/7115 ,  A61K31/712 ,  C12N15/11 ,  C12P19/34

CPC分类号： C07H21/02 ,  A23L33/13 ,  A61K31/711 ,  A61K31/7115 ,  A61K31/712 ,  C12N15/11 ,  C12P19/34 ,  C12Y207/07

摘要： Provided herein are methods and compositions for synthesizing 5′Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2′Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.

公开(公告)号：US20240300993A1

公开(公告)日：2024-09-12

申请号：US18574655

申请日：2022-06-28

申请人： SUMITOMO CHEMICAL COMPANY, LIMITED

发明人： Yuya MORIYAMA

IPC分类号： C07H21/02 ,  C07H1/00

CPC分类号： C07H21/02 ,  C07H1/00

摘要： The present invention provides a stable composition containing a nucleic acid oligomer having a phosphorothioate bond, a method for producing the same, and a method for efficiently producing the nucleic acid oligomer from the composition. The composition containing a nucleic acid oligomer having a phosphorothioate bond represented by Formula (1) (wherein symbols are as defined in the specification), an alkyl ammonium salt, a nitrile-based organic solvent, water, and an additive containing at least one compound selected from the group consisting of compounds represented by Formulae (3a) to (3h) is a stable composition.

公开(公告)号：US12077757B2

公开(公告)日：2024-09-03

申请号：US17063080

申请日：2020-10-05

申请人： Janssen Biopharma, Inc.

发明人： Sergei Gryaznov ,  Leonid Beigelman ,  Antitsa Dimitrova Stoycheva ,  Saul Martinez Montero ,  Jin Hong ,  Rajendra K. Pandey ,  Vivek Kumar Rajwanshi ,  Lakshmipathi Pandarinathan ,  Yi Jin ,  Bharat Baral

IPC分类号： C07H21/02 ,  A61K31/712 ,  A61K31/7125 ,  A61K47/54 ,  A61P31/20 ,  C12N15/11 ,  C12N15/113

CPC分类号： C12N15/1131 ,  A61K31/712 ,  A61K31/7125 ,  A61K47/549 ,  A61P31/20 ,  C07H21/02 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/314 ,  C12N2310/3145 ,  C12N2310/315 ,  C12N2310/3231 ,  C12N2310/341 ,  C12N2310/344 ,  C12N2310/351 ,  C12N2320/32 ,  C12N2310/321 ,  C12N2310/3525

摘要： Modified oligonucleotides comprising modifications at the 2′ and/or 3′ position(s) along with methods of making and use, e.g., against HBV are disclosed.

公开(公告)号：US12060385B2

公开(公告)日：2024-08-13

申请号：US18217783

申请日：2023-07-03

申请人： GUANGZHOU HENOVCOM BIOSCIENCE CO., LTD.

发明人： Jiancun Zhang ,  Lijun Zhang ,  Yiqian Zhou ,  Jiafeng Chen ,  Jufu Zhang ,  Suyong Li ,  Yanhui Liu ,  Chen Guo ,  Wanjun Tang ,  Feng Wu ,  Xiaoxi He ,  Qi Guo ,  Huixuan Chen ,  Kun Wang ,  Deyao Li

IPC分类号： C07H21/02 ,  C07H19/207 ,  C12P19/34

CPC分类号： C07H21/02 ,  C07H19/207 ,  C12P19/34

摘要： The present disclosure relates to compounds for RNA capping and uses thereof, which belong to the technical field of genetic engineering. Compounds of formula I can result in high levels of capping efficiency, and the capped mRNA exhibits a high transcription level and increased expression.

公开(公告)号：US12037642B2

公开(公告)日：2024-07-16

申请号：US17410504

申请日：2021-08-24

申请人： INDUSTRY-UNIVERSITY COOPERATION OF USW

发明人： Hyung Jong Jin ,  Hak Jin Lee

IPC分类号： C07H21/02 ,  C12Q1/6876

CPC分类号： C12Q1/6876 ,  C07H21/02 ,  C12Q2600/154 ,  C12Q2600/158

摘要： The RNA molecule of the present invention, which is based on a helix 73-derived RNA substrate and Erm interaction sites, may be used for screening candidate substances that inhibit methylation by Erm, developing variants thereof, and identifying the action mechanism, and may be used for suppressing the expression of antibiotic resistance.

公开(公告)号：US12037586B2

公开(公告)日：2024-07-16

申请号：US17859781

申请日：2022-07-07

申请人： Dicerna Pharmaceuticals, Inc.

发明人： Weimin Wang ,  Qingyi Li ,  Naim Nazef

IPC分类号： C12N15/113 ,  A61K31/7115 ,  A61K31/712 ,  A61K31/7125 ,  A61K45/06 ,  A61K47/54 ,  A61K47/60 ,  C07H19/10 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/113 ,  A61K31/7115 ,  A61K31/712 ,  A61K31/7125 ,  A61K45/06 ,  A61K47/549 ,  A61K47/60 ,  C07H19/10 ,  C07H21/02 ,  C07H21/04 ,  C12N2310/14 ,  C12N2310/31 ,  C12N2310/322 ,  C12N2310/344 ,  C12N2310/3515

摘要： Disclosed herein are oligonucleotides, such as nucleic acid inhibitor molecules, having a 4′-phosphate analog and methods of using the same, for example, to modulate the expression of a target gene in a cell. The phosphate analogs are bound to the 4′-carbon of the sugar moiety (e.g., a ribose or deoxyribose or analog thereof) of the 5′-terminal nucleotide of an oligonucleotide. Typically, the phosphate analog is an oxymethylphosphonate, where the oxygen atom of the oxymethyl group is bound to the 4′-carbon of the sugar moiety or analog thereof.

公开(公告)号：US12012432B2

公开(公告)日：2024-06-18

申请号：US16995477

申请日：2020-08-17

申请人： National Institute of Advanced Industrial Science and Technology

发明人： Yasuo Komatsu ,  Yu Hirano ,  Yasuhiro Mie

IPC分类号： C07H21/04 ,  A61K31/7088 ,  A61K31/713 ,  A61K48/00 ,  C07H21/02 ,  C12N15/00 ,  C12N15/09 ,  C12N15/113 ,  C12Q1/68 ,  C12Q1/6832

CPC分类号： C07H21/04 ,  A61K31/7088 ,  A61K31/713 ,  A61K48/00 ,  C07H21/02 ,  C12N15/00 ,  C12N15/09 ,  C12N15/113 ,  C12Q1/68 ,  C12Q1/6832 ,  C12N2310/141 ,  C12N2310/321

摘要： A nucleic acid complex includes a single-stranded nucleic acid and a cross-linked double-stranded nucleic acid including the first nucleic acid strand linked to at least one of the 5′ end and the 3′ end of the single-stranded nucleic acid and the second nucleic acid strand including a base sequence that is completely or sufficiently complementary to the first nucleic acid strand.

公开(公告)号：US20240165146A1

公开(公告)日：2024-05-23

申请号：US18340192

申请日：2023-06-23

申请人： Ionis Pharmaceuticals, Inc.

发明人： Nicholas J. Viney ,  Richard S. Geary ,  Yanfeng Wang ,  Zhengrong Yu ,  Rudy Gunawan

IPC分类号： A61K31/712 ,  A61K9/00 ,  A61K48/00 ,  A61P3/06 ,  C07H19/20 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/113

CPC分类号： A61K31/712 ,  A61K9/0019 ,  A61K48/0016 ,  A61K48/005 ,  A61K48/0083 ,  A61P3/06 ,  C07H19/20 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/113 ,  C12N2310/341 ,  C12N2310/3515

摘要： Provided herein are methods of administering gapmer oligomeric compounds with GalNAc conjugate groups to a human.

公开(公告)号：US11976092B2

公开(公告)日：2024-05-07

申请号：US16635312

申请日：2018-11-12

申请人： Ohio State Innovation Foundation ,  UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION

发明人： Peixuan Guo ,  Mario Vieweger ,  Xin Li ,  Sijin Guo ,  Hongran Yin ,  Xijun Piao ,  Yi Shu ,  Dan Shu ,  Mehdi Rajabi

IPC分类号： C07H21/02 ,  A61K45/06 ,  A61K47/54 ,  A61K47/55 ,  A61K51/06 ,  C12N15/115

CPC分类号： C07H21/02 ,  A61K47/549 ,  A61K47/55 ,  C12N15/115 ,  A61K45/06 ,  A61K51/06 ,  C12N2310/35 ,  C12N2310/351 ,  C12N2310/3519 ,  C12N2330/30

摘要： Disclosed herein are high Tm RNA nanostructures that can be composed of one or more modules or motifs to build RNA nanostructures with or without layers. The RNA nanostructures can have a core domain and three or more double-stranded arms and formulations thereof to conjugate high copy numbers of therapeutics, pH responsive or enzyme cleavable drug cargo. Also described herein is a design strategy for generation of synthetic RNA oligonucleotides that can self assemble into highly thermostable RNA structures. Also described herein are uses of the RNA nanostructures described herein.