公开(公告)号：US20050095650A1

公开(公告)日：2005-05-05

申请号：US10915017

申请日：2004-08-09

申请人： David Julius ,  Michael Caterina ,  Anthony Brake

发明人： David Julius ,  Michael Caterina ,  Anthony Brake

IPC分类号： A01K67/027 ,  A61K38/00 ,  A61K39/395 ,  A61P25/04 ,  C07K14/705 ,  C07K16/28 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/50 ,  G01N33/566 ,  G01N33/53

CPC分类号： C07K14/705 ,  A01K2217/05 ,  A61K38/00

摘要： The present invention features vanilloid receptor polypeptides and vanilloid receptor-related polypeptides, specifically the capsaicin receptor subtypes VR1 and VR2 (VRRP-1), as well as the encoding polynucleotide sequences. In related aspects the invention features expression vectors and host cells comprising such polynucleotides. In other related aspects, the invention features transgenic animals having altered capsaicin receptor expression, due to, for example, the presence of an exogenous wild-type or modified capsaicin receptor-encoding polynucleotide sequence. The present invention also relates to antibodies that bind specifically to a capsaicin receptor polypeptide, and methods for producing these polypeptides. Further, the invention provides methods for using capsaicin receptor, including methods for screening candidate agents for activity as agonists or antagonists of capsaicin receptor activity, as well as assays to determine the amount of a capsaicin receptor-activating agent in a sample. In other related aspects, the invention provides methods for the use of the capsaicin receptor for the diagnosis and treatment of human disease and painful syndromes.

摘要翻译： 本发明特征在于香草素受体多肽和香草素受体相关多肽，特别是辣椒素受体亚型VR1和VR2（VRRP-1）以及编码多核苷酸序列。 在相关方面，本发明的特征在于包含这种多核苷酸的表达载体和宿主细胞。 在其他相关方面，本发明的特征在于具有改变的辣椒素受体表达的转基因动物，这是由于例如外源野生型或修饰的辣椒素受体编码多核苷酸序列的存在。 本发明还涉及与辣椒素受体多肽特异性结合的抗体，以及制备这些多肽的方法。 此外，本发明提供了使用辣椒素受体的方法，包括用于筛选作为辣椒素受体活性的激动剂或拮抗剂的活性的候选药剂的方法，以及测定样品中辣椒素受体激活剂的量的测定法。 在其他相关方面，本发明提供了使用辣椒素受体来诊断和治疗人类疾病和疼痛综合征的方法。