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公开(公告)号:US20060289327A1
公开(公告)日:2006-12-28
申请号:US10540042
申请日:2003-12-23
申请人: David Knight , Larissa Pinnock
发明人: David Knight , Larissa Pinnock
IPC分类号: B65D85/00
摘要: An apparatus for the storage of a protein (20) comprising a first compartment (30) for storing the protein (20) and a second compartment (40) for storing an alkaline buffer (50), the second compartment (40) being in fluid communication with the first compartment (30) is described. In one preferential embodiment of the invention, the alkaline buffer comprises calcium ions which encourage the gelling of the protein (20). A method for the storage of a protein (20) is also described. The method comprises: a first step of placing the protein in a first storage compartment (30); a second step of exposing the protein (20) to an alkaline buffer (50); and a third step of maintaining the protein (20) in the alkaline atmosphere in the first storage compartment (30).
摘要翻译: 一种用于储存蛋白质(20)的装置,包括用于储存蛋白质(20)的第一隔室(30)和用于储存碱性缓冲液(50)的第二隔室(40),第二隔室(40)处于流体 描述与第一隔间(30)的连通。 在本发明的一个优选实施方案中,碱性缓冲剂包括促进蛋白质凝胶化的钙离子(20)。 还描述了用于存储蛋白质(20)的方法。 该方法包括:将蛋白质置于第一储藏室(30)中的第一步骤。 将蛋白质(20)暴露于碱性缓冲液(50)的第二步骤; 以及将蛋白质(20)保持在第一储藏室(30)中的碱性气氛中的第三步骤。
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公开(公告)号:US20050281859A1
公开(公告)日:2005-12-22
申请号:US11158842
申请日:2005-06-22
申请人: David Knight , Larissa Pinnock
发明人: David Knight , Larissa Pinnock
摘要: The invention utilizes the sol-gel transition behavior of certain proteins for forming objects. The invention is directed to a method and apparatus for forming an object from a feedstock made of a protein solution, the protein undergoing a sol-gel transition and comprising the following steps: a first step of adjusting the conditions to cause the feedstock to flow to form the object from with the protein substantially in the sol state, a second step of adjusting the conditions of the feedstock either to gel the feedstock or bring it close to the sol-gel transition point. Particularly preferred proteins are spidroin and fibroin, while one of the conditions of the feedstock will be adjusting the pH of the solution e.g., with a carboxylic acid.
摘要翻译: 本发明利用某些蛋白质的溶胶 - 凝胶转变行为形成物体。 本发明涉及一种从由蛋白质溶液制备的原料形成物体的方法和装置,所述原料是经历溶胶 - 凝胶转变的蛋白质,其包括以下步骤:调节条件以使原料流向 与基本上处于溶胶状态的蛋白质形成对象,第二步是调节原料的条件以使原料凝胶化或使其接近溶胶 - 凝胶转变点。 特别优选的蛋白质是蜘蛛蛋白和丝心蛋白,而原料的一种条件将调节溶液的pH,例如用羧酸。
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