公开(公告)号：US09714932B2

公开(公告)日：2017-07-25

申请号：US14110936

申请日：2012-04-13

申请人： Dhananjay T. Tambe ,  Jeffrey J. Fredberg ,  James Butler ,  Xavier Trepat

发明人： Dhananjay T. Tambe ,  Jeffrey J. Fredberg ,  James Butler ,  Xavier Trepat

IPC分类号： G01N33/483 ,  G06T7/246 ,  G06K9/00

CPC分类号： G01N33/4833 ,  G06K9/00134 ,  G06K9/0014 ,  G06T7/246 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30204

摘要： Disclosed are systems, apparatus, devices and methods, including a method that includes determining traction forces exerted by a cellular monolayer on a substrate on which the monolayer is placed, and determining internal forces within and between cells of the monolayer based on the determined traction forces. In some embodiments, determining the internal forces of the cellular monolayer may include determining internal stresses within the cellular monolayer that act to balance the determined traction forces over at least part of the cellular monolayer. In some embodiments, determining of the internal stresses may also include setting boundary conditions at a boundary determined based on an optical field of view of an observed section of the monolayer.

公开(公告)号：US20140212909A1

公开(公告)日：2014-07-31

申请号：US14110936

申请日：2012-04-13

申请人： Dhananjay T. Tambe ,  Jeffrey J. Fredberg ,  James Butler ,  Xavier Trepat

发明人： Dhananjay T. Tambe ,  Jeffrey J. Fredberg ,  James Butler ,  Xavier Trepat

IPC分类号： G01N33/483 ,  G06T7/20 ,  G06K9/00

CPC分类号： G01N33/4833 ,  G06K9/00134 ,  G06K9/0014 ,  G06T7/246 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30204

摘要： Disclosed are systems, apparatus, devices and methods, including a method that includes determining traction forces exerted by a cellular monolayer on a substrate on which the monolayer is placed, and determining internal forces within and between cells of the monolayer based on the determined traction forces. In some embodiments, determining the internal forces of the cellular monolayer may include determining internal stresses within the cellular monolayer that act to balance the determined traction forces over at least part of the cellular monolayer. In some embodiments, determining of the internal stresses may also include setting boundary conditions at a boundary determined based on an optical field of view of an observed section of the monolayer.

摘要翻译： 公开了一种系统，装置，装置和方法，包括一种方法，其包括确定细胞单层在其上放置单层的基底上施加的牵引力，以及基于所确定的牵引力确定单层内和内之间的内力 。 在一些实施方案中，确定细胞单层的内部力可以包括确定细胞单层内的内部应力，其用于平衡在至少部分细胞单层上确定的牵引力。 在一些实施例中，内部应力的确定还可以包括在基于单层观测部分的光学视场确定的边界处设置边界条件。