公开(公告)号：US11327079B2

公开(公告)日：2022-05-10

申请号：US16627710

申请日：2018-12-12

Applicant: Diatech Korea Co., Ltd.

Inventor： Kye Shin Park ,  Joon Sang Park ,  Eun Hee Lee ,  Dong Hwi Hwang ,  In Jung Ji ,  Jae Woo Roh ,  Jin Sung Ahn ,  Eun-Jeong Yoon ,  Ji Hye Ko

IPC: G01N33/68 ,  C12N9/86 ,  C12Q1/34 ,  C12Q1/04 ,  C12Q1/06

Abstract: The present invention relates to a method of directly detecting, using a mass-spectrometry method, whether a microorganism contained in a sample is resistant to antibiotics, and a kit for detection used therewith. More particularly, the present invention relates to a method and kit for directly detecting an antibiotic hydrolase secreted by a microorganism resistant to antibiotics, thereby directly determining whether the microorganism is resistant to antibiotics. According to the present invention, it is possible to very simply and immediately confirm whether a specific strain is resistant to antibiotics in the field. In particular, a complicated pretreatment process such as proteolysis is not performed, and a complicated identification process of calibrating and then combining the obtained results is not performed. Accordingly, it is possible to realize a method of easily confirming whether antibiotic resistance occurs in just a dozen minutes, compared to a conventional technology in which it takes several days to confirm whether antibiotic resistance occurs, and a simple diagnostic kit used therewith.