公开(公告)号：US06241894B1

公开(公告)日：2001-06-05

申请号：US08948357

申请日：1997-10-10

申请人： Donald David Briggs ,  Srikanth Ranga Chary ,  Shang-Chih David Jen ,  Richard Merrill Schwartz

发明人： Donald David Briggs ,  Srikanth Ranga Chary ,  Shang-Chih David Jen ,  Richard Merrill Schwartz

IPC分类号： B01D3506

CPC分类号： B03C1/035 ,  Y10S977/888 ,  Y10S977/902

摘要： The separation device of the invention is directed to a container having an interior surface defining a channel. The container further has an inlet and an outlet. On the interior surface of the channel are pole tips which may be in a sawtooth configuration having sharp angles facing the interior of the channel that generate a high gradient magnetic field in the channel. Within the channel may be incorporated separating material. The separating material eliminates the direct contact of cells with the magnetic pole material. The separating material, as well as the sawtooth pole tips also serves the purpose of creating a field gradient across the entire container to avoid the problem of zero field gradient in the center of the container where the velocity is greatest, and where more cells flow. The separating material is designed to cause a substantially unobstructed flow of medium through the channel so that unlabeled substances are not trapped in the separating material.

摘要翻译： 本发明的分离装置针对具有限定通道的内表面的容器。 容器还具有入口和出口。 在通道的内表面上是极尖，其可以是锯齿形结构，其具有面向通道内部的锐角，在通道中产生高梯度磁场。 通道内可以加入分离材料。 分离材料消除了电池与磁极材料的直接接触。 分离材料以及锯齿极尖也用于在整个容器上形成场梯度的目的，以避免在容器中心处的零场梯度的问题，其中速度最大，并且更多的单元流动。 分离材料被设计成使介质通过通道基本上无障碍地流动，使得未标记的物质不被捕获在分离材料中。

公开(公告)号：US5728581A

公开(公告)日：1998-03-17

申请号：US482259

申请日：1995-06-07

申请人： Richard Merrill Schwartz ,  Sean Newton Tucker ,  Srikanth Ranga Chary ,  Suzanne Chang Kuo

发明人： Richard Merrill Schwartz ,  Sean Newton Tucker ,  Srikanth Ranga Chary ,  Suzanne Chang Kuo

IPC分类号： C12M3/00 ,  C12M3/06 ,  C12N5/0789 ,  C12N5/00 ,  C12M1/22 ,  C12N5/02

CPC分类号： C12M25/06 ,  C12M23/02 ,  C12M23/04 ,  C12M23/24 ,  C12M25/02 ,  C12M41/32 ,  C12N5/0647 ,  C12N2500/02 ,  C12N2500/90 ,  C12N2501/125 ,  C12N2501/22 ,  C12N2501/23

摘要： The present invention provides methods and bioreactors for expanding stem cells in a population of cells substantially enriched in hematopoietic stem cells and substantially free of stromal cells. The method comprising the steps of inoculating the population of cells in an expansion container in a volume of suitable medium such that the cell density is at least about 5,000 cells/1 mL and at an initial oxygen concentration of less than 8%; adding an effective amount of at least one cytokine to cause stem cell expansion; culturing the cells under suitable conditions such that the cells condition the medium; increasing the oxygen concentration to about 20%; exchanging the medium at a rate which allows expansion of the stem cells; and culturing the cells under conditions such that the stem cells are expanded. The present invention also provides a bioreactor constructed to accomodate the operational requirements for stroma-free stem cell expansion.

摘要翻译： 本发明提供用于在基本上富含造血干细胞并基本上不含基质细胞的细胞群中扩增干细胞的方法和生物反应器。 该方法包括以下步骤：将细胞群接种在一定体积的合适培养基中的膨胀容器中，使得细胞密度为至少约5,000个细胞/ mL，初始氧浓度小于8％; 加入有效量的至少一种细胞因子以引起干细胞扩张; 在合适的条件下培养细胞，使细胞调节培养基; 将氧浓度提高到约20％; 以允许干细胞扩增的速率交换培养基; 并在使干细胞扩大的条件下培养细胞。 本发明还提供了一种生物反应器，其被构造为适应无基质干细胞扩增的操作要求。