公开(公告)号：US09163214B2

公开(公告)日：2015-10-20

申请号：US13379651

申请日：2010-05-04

申请人： Dongmei Lai ,  Weiwei Cheng ,  Lihe Guo ,  Tianjin Liu ,  Lizhen Jiang ,  Qin Huang

发明人： Dongmei Lai ,  Weiwei Cheng ,  Lihe Guo ,  Tianjin Liu ,  Lizhen Jiang ,  Qin Huang

IPC分类号： C12N5/0735 ,  C12N5/074 ,  C12N5/095

CPC分类号： C12N5/0606 ,  C12N5/0607 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2502/025

摘要： In the field of biological technology, a stem cell culture method is provided. The method includes preparing an amniotic epithelial cell feeder layer that is not treated to lose the division ability; and seeding the stem cells onto the amniotic epithelial cell feeder layer, and culturing in a culture medium. The stem cell culture method according to the present invention does not require the treatment of the feeder layer cells to lose the division ability, and is thus simple and safe, thereby effectively solving the problem of contamination caused by animal-derived ingredients in culture of human stem cells at present, greatly reducing the culture cost of the stem cells, and providing a safe, effective, and inexpensive stem cell culture method for the industrialization of the stem cells in the future.

摘要翻译： 在生物技术领域，提供了干细胞培养方法。 该方法包括制备未被处理以丧失分裂能力的羊膜上皮细胞饲养层; 并将干细胞接种到羊膜上皮细胞饲养层上，并在培养基中培养。 根据本发明的干细胞培养方法不需要对饲养层细胞的处理以失去分裂能力，因此简单安全，从而有效地解决了动物来源的成分在人类培养中引起的污染问题 目前干细胞大大降低了干细胞的培养成本，为将来干细胞工业化提供了一种安全，有效，廉价的干细胞培养方法。

公开(公告)号：US20120122213A1

公开(公告)日：2012-05-17

申请号：US13379651

申请日：2010-05-04

申请人： Dongmei Lai ,  Weiwei Cheng ,  Lihe Guo ,  Tianjin Liu ,  Lizhen Jiang ,  Qin Huang

发明人： Dongmei Lai ,  Weiwei Cheng ,  Lihe Guo ,  Tianjin Liu ,  Lizhen Jiang ,  Qin Huang

IPC分类号： C12N5/071 ,  C12N5/0735

CPC分类号： C12N5/0606 ,  C12N5/0607 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2502/025

摘要： In the field of biological technology, a stem cell culture method is provided. The method includes preparing an amniotic epithelial cell feeder layer that is not treated to lose the division ability; and seeding the stem cells onto the amniotic epithelial cell feeder layer, and culturing in a culture medium. The stem cell culture method according to the present invention does not require the treatment of the feeder layer cells to lose the division ability, and is thus simple and safe, thereby effectively solving the problem of contamination caused by animal-derived ingredients in culture of human stem cells at present, greatly reducing the culture cost of the stem cells, and providing a safe, effective, and inexpensive stem cell culture method for the industrialization of the stem cells in the future.

摘要翻译： 在生物技术领域，提供了干细胞培养方法。 该方法包括制备未被处理以丧失分裂能力的羊膜上皮细胞饲养层; 并将干细胞接种到羊膜上皮细胞饲养层上，并在培养基中培养。 根据本发明的干细胞培养方法不需要对饲养层细胞的处理以失去分裂能力，因此简单安全，从而有效地解决了动物来源的成分在人类培养中引起的污染问题 目前干细胞大大降低了干细胞的培养成本，为将来干细胞工业化提供了一种安全，有效，廉价的干细胞培养方法。