公开(公告)号：US20080268445A1

公开(公告)日：2008-10-30

申请号：US11871956

申请日：2007-10-12

申请人： Douglas C. Wallace ,  Grant MacGregor ,  Katrina Waymire ,  Shawn E. Levy ,  James E. Sligh ,  Jason E. Kokoszka

发明人： Douglas C. Wallace ,  Grant MacGregor ,  Katrina Waymire ,  Shawn E. Levy ,  James E. Sligh ,  Jason E. Kokoszka

IPC分类号： C12Q1/68 ,  A01K67/027 ,  C12N5/18 ,  C12Q1/02 ,  G01N33/68

CPC分类号： C07K14/47 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  C12N15/8509 ,  C12N2517/02 ,  C12N2800/30

摘要： Described are methods for inactivating adenine nucleotide transporter proteins in specific tissues of a transgenic nonhuman animal using a conditional knockin/knockout technology such as the Cre-LoxP, Flip-FLP recombinase, or Tet-on/off technologies. Specifically, the Ant2 gene is functionally inactivated in a mouse in liver, with or without the concurrent inactivation of the Ant1 gene. The result is an animal in which the Ant2 gene and accompanying ANT 2 protein is absent in one or more tissues, either in the presence or absence of the Ant1 gene and accompanying protein. The resulting animals, cells, mitochondria, and subcelluar fractions such as the mitochondrial permeability transition pore can then be used to identify agents that affect animal and/or subcellular function via a direct or indirect interaction with the ANT2 protein and/or its Ant2 gene.

摘要翻译： 描述了使用诸如Cre-LoxP，Flip-FLP重组酶或Tet-on / off技术的条件敲入/敲除技术使转基因非人动物的特定组织中的腺嘌呤核苷酸转运蛋白失活的方法。 具体来说，Ant2基因在肝脏中的小鼠中功能失活，有或没有同时灭活Ant1基因。 结果是在存在或不存在Ant1基因和伴随的蛋白质的情况下，一种或多种组织中不存在Ant2基因和伴随的ANT2蛋白的动物。 所得到的动物，细胞，线粒体和亚细胞级分如线粒体通透性转换孔可用于通过与ANT2蛋白和/或其Ant2基因的直接或间接相互作用来鉴定影响动物和/或亚细胞功能的试剂。