公开(公告)号：US07585970B2

公开(公告)日：2009-09-08

申请号：US10652049

申请日：2003-08-30

申请人： Douglas J Dellinger ,  Geraldine F Dellinger ,  John S Hargreaves

发明人： Douglas J Dellinger ,  Geraldine F Dellinger ,  John S Hargreaves

IPC分类号： C07H21/00

CPC分类号： C07H21/00 ,  Y02P20/55

摘要： A method for synthesizing a polynucleotide is disclosed. The method comprises providing a functionalized support comprising a triaryl methyl linker bound to the solid support through a substitution on one of the aryl groups, a nucleotide chain bound to the central methyl carbon of the triaryl methyl group and a nucleoside with a reactive site hydroxyl. The method further provides contacting that functionalized support with a precursor having a hydroxyl protecting group and a phosphorous derivative reactive group to produce internucleotide linkage. The resulting compound is then treated to both remove the hydroxyl protecting group on the precursor, and to oxidize the internucleotide bond.

摘要翻译： 公开了一种合成多核苷酸的方法。 该方法包括提供官能化载体，其包含通过在一个芳基上取代而与固体支持物结合的三芳基甲基接头，与三芳基甲基的中心甲基结合的核苷酸链和具有反应性位点羟基的核苷。 该方法还使官能化载体与具有羟基保护基团和磷衍生物反应性基团的前体接触以产生核苷酸间键合。 然后处理所得化合物以去除前体上的羟基保护基团并氧化核苷酸间键。