公开(公告)号：US07138518B1

公开(公告)日：2006-11-21

申请号：US09714579

申请日：2000-11-16

申请人： Douglas T. Gjerde ,  Robert M. Haefele ,  Paul D. Taylor ,  Christopher P. Hanna ,  Alezander I. Kuklin ,  David P. Hornby

发明人： Douglas T. Gjerde ,  Robert M. Haefele ,  Paul D. Taylor ,  Christopher P. Hanna ,  Alezander I. Kuklin ,  David P. Hornby

IPC分类号： C07H21/00 ,  C12Q1/68 ,  B01D15/00 ,  B01D15/08

CPC分类号： C12N15/101

摘要： In one aspect, the invention provides a method for separating a mixture of polynucleotides, such as DNA or RNA, including (a) applying the mixture to a polymeric separation medium having non-polar surfaces, wherein the surfaces are characterized by being substantially free from multivalent cations, such as metal ions, which are free to interfere with polynucleotide separation, and (b) eluting the mixture with a mobile phase containing organic solvent and counter ion agent. In the separation of single-stranded polynucleotides, improved separation is obtained at a temperature effective to fully denature secondary structure within the polynucleotides.

摘要翻译： 一方面，本发明提供了分离多核苷酸（例如DNA或RNA）的混合物的方法，包括（a）将该混合物施用于具有非极性表面的聚合物分离介质，其中所述表面的特征在于基本上不含 可自由干扰多核苷酸分离的多价阳离子，例如金属离子，和（b）用含有机溶剂和抗衡离子剂的流动相洗脱该混合物。 在单链多核苷酸的分离中，在有效使多核苷酸内完全变性二级结构的温度下获得改进的分离。