公开(公告)号：US20150099648A1

公开(公告)日：2015-04-09

申请号：US14505060

申请日：2014-10-02

Applicant: Dow AgroSciences LLC

Inventor： Sivarama Reddy CHENNAREDDY ,  Dayakar PAREDDY ,  Jayakumar Pon SAMUEL ,  Rodrigo SARRIA-MILAN ,  Toby CICAK

IPC: G01N21/64 ,  G01N33/68 ,  G01N33/00

CPC classification number: C12N15/8201

Abstract: The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants by incorporating a selection agent within rooting medium used in tissue culture during the soybean transformation process. The soybean germline transformants are selected from a population of soybean transformants which are comprised of a combination of non-germline and germline soybean transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are identified and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the soybean transformation process.

Abstract translation: 本公开部分地涉及通过在大豆转化过程中在组织培养中使用的生根培养基中掺入选择剂来从大豆转化体群体中鉴定大豆种系转化体的方法。 大豆种系转化体选自由非种系和种系大豆转化体的组合组成的大豆转化体群体。 大豆非种系转化体在转化过程的早期被鉴定和消除。 鉴定并选择大豆种系转化体用于培养成熟大豆植物。 该方法适用于大豆转化过程早期筛选和获得大豆种系转化体。