公开(公告)号：US20220174981A1

公开(公告)日：2022-06-09

申请号：US17534663

申请日：2021-11-24

申请人： DuPont Nutrition Biosciences APS

发明人： Karsten Matthias KRAGH ,  Ernst MEINJOHANNS ,  Andrei MIASNIKOV ,  Maria MA ,  Thomas EISELE ,  Svend HAANING ,  Peter Edvard DEGN ,  Steffen Yde BAK

IPC分类号： A23K10/14 ,  A23K50/75 ,  A23K50/30 ,  C12P21/06 ,  C12N9/48 ,  A23K20/147 ,  A23J3/34

摘要： A method for the production of a hydrolysate comprising: (a) admixing at least one protein or a portion thereof with: (A) at least one endoprotease; and (B) (a′) at least one proline tolerant tripeptidyl peptidase or fermentate comprising a proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at P1; and synthetic amino acids at P1; or (b′) at least one proline tolerant tripeptidyl peptidase having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at PV; and synthetic amino acids at PV; and (b) recovering the hydrolysate. The invention also relates to methods for producing a hydrolysate comprising the use of an endoprotease an exo-tripeptidyl peptidase of the S53 family and an aminopeptidase, to uses of a proline tolerant tripeptidyl peptidase, compositions, food and/or feed additive compositions comprising the same, as well as hydrolysates and uses of proline tolerant tripeptidyl peptidases.

公开(公告)号：US20220136027A1

公开(公告)日：2022-05-05

申请号：US17433767

申请日：2020-02-25

申请人： DUPONT NUTRITION BIOSCIENCES APS

发明人： Peter Edvard DEGN ,  Xiaogang GU ,  Karsten Matthias KRAGH ,  Robin Anton SORG ,  Steffen Yde BAK ,  Svend HAANING ,  Xinyue TANG ,  Helong HAO ,  Marc Anton Bernhard KOLKMAN

IPC分类号： C12P21/06 ,  A23J3/34 ,  A23J3/18

摘要： The present invention related to a method for preparing a protein hydrolysate from a proteinaceous material by contacting the material with a proteolytic enzyme mixture having a proline specific exopeptidase. In particular, the proline specific exopeptidase is an aminopeptidase specific for at the five amino acid N-terminal sequence X-Pro-Gln-Glv-Pro-, where X is any amino acid. The present invention also relates to use of the aminopeptidase with a second exopeptidase and an endopeptidase.

公开(公告)号：US20170325479A1

公开(公告)日：2017-11-16

申请号：US15521245

申请日：2015-10-23

申请人： DUPONT NUTRITION BIOSCIENCES APS

发明人： Karsten Matthias KRAGH ,  Ernst MEINJOHANNS ,  Andrei MIASNIKOV ,  Maria MA ,  Thomas EISELE ,  Svend HAANING ,  Peter Edvard DEGN ,  Steffen Yde BAK

IPC分类号： A23J3/34 ,  A23K20/147 ,  C12N9/48 ,  A23K50/75 ,  A23K50/30 ,  C12P21/06 ,  A23K10/14

CPC分类号： A23K10/14 ,  A23J3/34 ,  A23K20/147 ,  A23K50/30 ,  A23K50/75 ,  C12N9/485 ,  C12P21/06 ,  C12Y304/14 ,  C12Y304/14009

摘要： A method for the production of a hydrolysate comprising: (a) admixing at least one protein or a portion thereof with: (A) at least one endoprotease; and (B) (a′) at least one proline tolerant tripeptidyl peptidase or fermentate comprising a proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at P1; and synthetic amino acids at P1; or (b′) at least one proline tolerant tripeptidyl peptidase having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at PV; and synthetic amino acids at PV; and (b) recovering the hydrolysate. The invention also relates to methods for producing a hydrolysate comprising the use of an endoprotease an exo-tripeptidyl peptidase of the S53 family and an aminopeptidase, to uses of a proline tolerant tripeptidyl peptidase, compositions, food and/or feed additive compositions comprising the same, as well as hydrolysates and uses of proline tolerant tripeptidyl peptidases.