公开(公告)号：US20150337330A1

公开(公告)日：2015-11-26

申请号：US14430029

申请日：2013-09-20

申请人： E.I. DUPONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL, INC. ,  PURDUE RESEARCH FOUNDATION

发明人： John Lindsey Flexner ,  Gurmukh S. Johal ,  Dilbag S. Multani ,  James K. Presnail ,  Scott V. Tingey

IPC分类号： C12N15/82 ,  A01B79/02

CPC分类号： C12N15/8286 ,  A01B79/02 ,  A01H5/10 ,  C07K14/415

摘要： Trap and/or refuge crops and methods of use in managing corn rootworm and other insect pests of maize are provided. Some methods involve using trap crops comprising plants with enhanced susceptibility to one or more insect pests to lure and kill insect pests. Other methods involve using refuge crops comprising plants with enhanced susceptibility to one or more insect pests to monitor insect pest populations in an area or to promote mating between insecticide resistant and insecticide non-resistant insects.

摘要翻译： 提供了陷阱和/或避难作物以及管理玉米根虫和其他玉米害虫的方法。 一些方法涉及使用含有对一种或多种害虫诱导和杀死害虫的易感性增强的植物的捕捞作物。 其他方法涉及使用包含具有对一种或多种害虫的敏感性增强的植物的避难作物来监测某一地区的害虫种群或促进杀虫剂抗性和杀虫剂非抗性昆虫之间的交配。

公开(公告)号：US20150275228A1

公开(公告)日：2015-10-01

申请号：US14430002

申请日：2013-09-20

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  PURDUE RESEARCH FOUNDATION

发明人： Dilbag S. Multani ,  Gurmukh S. Johal ,  Bala K.P. Venkata

IPC分类号： C12N15/82 ,  C12Q1/68

CPC分类号： C12N15/8286 ,  A01H1/04 ,  A01H5/10 ,  C07K14/415 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  Y02A40/162

摘要： Methods and compositions for increasing a plant's resistance to an insect pest such as the corn rootworm are provided. Methods are provided for overexpression of Crw1, or variants thereof, in a host plant or plant cell to increase resistance to an insect pest in a plant such as maize. Moreover, methods are provided for identifying variants of Crw1 that when incorporated into a plant via transgenic or traditional breeding means increase resistance to an insect pest in a plant such as maize.

摘要翻译： 提供了增加植物对诸如玉米根虫等害虫的抗性的方法和组合物。 提供了用于在宿主植物或植物细胞中过度表达Crw1或其变体的方法，以增加对植物如玉米中的害虫的抗性。 此外，提供用于鉴定Crw1的变体的方法，当通过转基因或传统育种将其掺入植物时，增加对诸如玉米的植物中的害虫的抗性。

公开(公告)号：US09944949B2

公开(公告)日：2018-04-17

申请号：US14430002

申请日：2013-09-20

申请人： Pioneer Hi-Bred International, Inc. ,  Purdue Research Foundation

发明人： Dilbag S. Multani ,  Gurmukh S. Johal ,  Bala K. P. Venkata

IPC分类号： C12N15/82 ,  A01H1/04 ,  A01H5/10 ,  C07K14/415 ,  C12Q1/68

CPC分类号： C12N15/8286 ,  A01H1/04 ,  A01H5/10 ,  C07K14/415 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  Y02A40/162

摘要： Methods and compositions for increasing a plant's resistance to an insect pest such as the corn rootworm are provided. Methods are provided for overexpression of Crw1, or variants thereof, in a host plant or plant cell to increase resistance to an insect pest in a plant such as maize. Moreover, methods are provided for identifying variants of Crw1 that when incorporated into a plant via transgenic or traditional breeding means increase resistance to an insect pest in a plant such as maize.

公开(公告)号：US09938537B2

公开(公告)日：2018-04-10

申请号：US14430017

申请日：2013-09-20

申请人： E. I. du Pont de Nemours and Company ,  Pioneer Hi-Bred International, Inc. ,  Purdue Research Foundation

发明人： Gurmukh S. Johal ,  Bailin Li ,  Dilbag S. Multani

IPC分类号： C12N15/82 ,  A01H1/04 ,  A01H5/10 ,  C07K14/415

CPC分类号： C12N15/8286 ,  A01H1/04 ,  A01H5/10 ,  C07K14/415 ,  Y02A40/162 ,  Y02A40/166

摘要： Methods and compositions for increasing a plant's resistance to an insect pest such as the corn rootworm are provided. Methods are provided for overexpression of Crw2, or variants thereof, in a host plant or plant cell to increase resistance to an insect pest in a plant such as maize. Methods are also provided for identifying variants of Crw2 that when incorporated into a plant via transgenic or traditional breeding means increase resistance to an insect pest in a plant such as maize. Also provided are methods for increasing resistance by overexpressing Crw1 and Crw2.

公开(公告)号：US10570413B2

公开(公告)日：2020-02-25

申请号：US14430029

申请日：2013-09-20

申请人： PURDUE RESEARCH FOUNDATION ,  E.I. du Pont de Nemours and Company ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： John Lindsey Flexner ,  Gurmukh S. Johal ,  Dilbag S. Multani ,  James K. Presnail ,  Scott V. Tingey

IPC分类号： C12N15/82 ,  A01B79/02

摘要： Trap and/or refuge crops and methods of use in managing corn rootworm and other insect pests of maize are provided. Some methods involve using trap crops comprising plants with enhanced susceptibility to one or more insect pests to lure and kill insect pests. Other methods involve using refuge crops comprising plants with enhanced susceptibility to one or more insect pests to monitor insect pest populations in an area or to promote mating between insecticide resistant and insecticide non-resistant insects.

公开(公告)号：US10595479B2

公开(公告)日：2020-03-24

申请号：US15305709

申请日：2015-04-24

申请人： Purdue Research Foundation

发明人： Brian Dilkes ,  Gurmukh S. Johal

IPC分类号： A01H1/06

摘要： Presented herein are methods for creating a desired genetic variant at a high efficiency from a seed. In one aspect, a method for creating a desired genetic variant at a high efficiency from a seed is presented. The method involves subjecting isolated pollen to a chemical mutagen to produce a first generation seed, planting the first generation seed in (n) plots to produce first generation plants, recovering mutagenized pollen from the first generation plants, pollinating a female parent with the recovered mutagenized pollen to produce a next generation seed, subjecting pollen from the next generation seed to mutagenesis to pollinate the first generation plant, resulting in a new next generation seed, planting the new next generation seed to produce a new next generation plant, and subjecting pollen from the new next generation plant to mutagenesis to pollinate the new next generation plant.