公开(公告)号：US10570413B2

公开(公告)日：2020-02-25

申请号：US14430029

申请日：2013-09-20

申请人： PURDUE RESEARCH FOUNDATION ,  E.I. du Pont de Nemours and Company ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： John Lindsey Flexner ,  Gurmukh S. Johal ,  Dilbag S. Multani ,  James K. Presnail ,  Scott V. Tingey

IPC分类号： C12N15/82 ,  A01B79/02

摘要： Trap and/or refuge crops and methods of use in managing corn rootworm and other insect pests of maize are provided. Some methods involve using trap crops comprising plants with enhanced susceptibility to one or more insect pests to lure and kill insect pests. Other methods involve using refuge crops comprising plants with enhanced susceptibility to one or more insect pests to monitor insect pest populations in an area or to promote mating between insecticide resistant and insecticide non-resistant insects.

公开(公告)号：US20150337330A1

公开(公告)日：2015-11-26

申请号：US14430029

申请日：2013-09-20

申请人： E.I. DUPONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL, INC. ,  PURDUE RESEARCH FOUNDATION

发明人： John Lindsey Flexner ,  Gurmukh S. Johal ,  Dilbag S. Multani ,  James K. Presnail ,  Scott V. Tingey

IPC分类号： C12N15/82 ,  A01B79/02

CPC分类号： C12N15/8286 ,  A01B79/02 ,  A01H5/10 ,  C07K14/415

摘要： Trap and/or refuge crops and methods of use in managing corn rootworm and other insect pests of maize are provided. Some methods involve using trap crops comprising plants with enhanced susceptibility to one or more insect pests to lure and kill insect pests. Other methods involve using refuge crops comprising plants with enhanced susceptibility to one or more insect pests to monitor insect pest populations in an area or to promote mating between insecticide resistant and insecticide non-resistant insects.

摘要翻译： 提供了陷阱和/或避难作物以及管理玉米根虫和其他玉米害虫的方法。 一些方法涉及使用含有对一种或多种害虫诱导和杀死害虫的易感性增强的植物的捕捞作物。 其他方法涉及使用包含具有对一种或多种害虫的敏感性增强的植物的避难作物来监测某一地区的害虫种群或促进杀虫剂抗性和杀虫剂非抗性昆虫之间的交配。

公开(公告)号：US20160025697A1

公开(公告)日：2016-01-28

申请号：US14776158

申请日：2014-03-10

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. DUPONT DE NEMOURS & COMPANY

发明人： Jessie L. Alt ,  Kevin A. Kriss ,  Thomas Morris ,  James K. Presnail ,  Molly Ryan-Mahmutagic

IPC分类号： G01N33/00

CPC分类号： G01N33/0098 ,  A01H1/04

摘要： Methods are provided for evaluating the insect resistance of a plant of interest. Specifically, methods are provided for high-throughput screening of soybean plants for resistance to kudzu bugs and stink bugs, including the brown marmorated stink bug and the southern green stink bug. In some embodiments, infesting emergent soybean seedlings with second instar stink bug nymphs and maintaining the nymphs in a closed environment with the seedling for only about 7 days allows evaluation of the stink bug resistance of a soybean variety of interest. Also provided are insect resistant plants, particularly plants resistant to kudzu bugs and stink bugs, as well as seeds produced by the resistant plants. Plants disclosed herein can be used to transfer the resistant trait into plant lines of interest.

摘要翻译： 提供了评估感兴趣植物的抗虫性的方法。 具体来说，提供了大豆植物高通量筛选方法，用于抵抗葛虫和臭臭虫，包括棕色马蹄臭虫和南方绿色臭虫。 在一些实施方案中，具有第二龄臭蝽若虫的侵袭性大豆幼苗并且在封闭环境中将幼苗保持在约7天的幼苗中，可以评估所关心的大豆品种的臭椿抗性。 还提供了抗虫植物，特别是抗葛虫和臭虫的植物，以及由抗性植物产生的种子。 本文公开的植物可以用于将抗性性状转移到感兴趣的植物系中。

公开(公告)号：US20150257389A1

公开(公告)日：2015-09-17

申请号：US14657795

申请日：2015-03-13

申请人： Pioneer Hi-Bred International, Inc. ,  E. I. du Pont de Nemours and Company

发明人： Xu Hu ,  James K. Presnail ,  Nina Richtman ,  Lisa Procyk

IPC分类号： A01N57/16 ,  C12N15/82

CPC分类号： C12N15/8218 ,  A01N57/20 ,  C12N15/8286 ,  Y02A40/162

摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: disclosed herein, (but not including the forward and reverse primers.) or active variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

摘要翻译： 提供了使用沉积元件的方法和组合物，当由有害生物摄取的诸如鞘翅目植物病虫害（Diaprotica plant pest）的害虫时，会降低害虫中靶序列的表达。 本发明提供了本文所公开的任何一个（但不包括正向和反向引物）或其活性变体及其片段或其互补序列的任何一种或多种靶基因，其中一个或多个 目标害虫中更多的序列控制害虫（即具有杀虫活性）。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。