公开(公告)号：US20180067076A1

公开(公告)日：2018-03-08

申请号：US15546671

申请日：2016-01-08

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Yitao LONG ,  Chan CAO ,  Yongxu HU

IPC: G01N27/447 ,  C12Q1/68 ,  G01N33/487

Abstract: A preparation method for an aerolysin nanopore in this disclosure comprises the following steps; (1) pretreatment of an aerolysin; (2) preparation of a lipid bilayer membrane by pulling process; (3) forming of the aerolysin nanopore: the aerolysin nanopore is obtained at a current of 50±5 pA. The aerolysin nanopore prepared in the invention is structurally stable and has a high resolution with the whole internal cavity carried with a positive charge, can be used for detection without modification and is easily operated. Further, the aerolysin nanopore can be applied in DNA sequencing, DNA damage and Micro-RNA detection.