公开(公告)号：US20190274299A1

公开(公告)日：2019-09-12

申请号：US16347429

申请日：2017-11-22

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Meijin Guo ,  Jiarui Li ,  Zejian Wang ,  Yuxia Chen ,  Yingping Zhuang

IPC: A01N1/02 ,  G01N27/22

Abstract: The present disclosure relates to the application of a viable cell monitor to the cryopreservation of plant cells. The viable cell monitor is, for the first time, used in a method for rapidly evaluating the effect of cell freezing-preservation. The effect of a cryoprotectant on cell preservation is evaluated through the change in the capacitance of viable cells before and after cell freezing and resuscitation. Viable cell electrodes can provide a quick and accurate characterization of viable cell concentrations. Combining the viable cell capacitance with the detection result of 2,3,4-triphenyl tetrazolium chloride (TTC), a rapid and quantitative evaluation of the effect of a freezing method on cell preservation can be achieved, thus overcoming the low effectiveness and non-quantification of previous cell preservation methods. Compared with traditional methods, the method of the present disclosure can improve the comprehensive score of cell cryopreservation by 5 times or more.

公开(公告)号：US11291200B2

公开(公告)日：2022-04-05

申请号：US16347429

申请日：2017-11-22

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Meijin Guo ,  Jiarui Li ,  Zejian Wang ,  Yuxia Chen ,  Yingping Zhuang

IPC: A01N1/02 ,  G01N27/22

Abstract: The present disclosure relates to the application of a viable cell monitor to the cryopreservation of plant cells. The viable cell monitor is, for the first time, used in a method for rapidly evaluating the effect of cell freezing-preservation. The effect of a cryoprotectant on cell preservation is evaluated through the change in the capacitance of viable cells before and after cell freezing and resuscitation. Viable cell electrodes can provide a quick and accurate characterization of viable cell concentrations. Combining the viable cell capacitance with the detection result of 2,3,4-triphenyl tetrazolium chloride (TTC), a rapid and quantitative evaluation of the effect of a freezing method on cell preservation can be achieved, thus overcoming the low effectiveness and non-quantification of previous cell preservation methods. Compared with traditional methods, the method of the present disclosure can improve the comprehensive score of cell cryopreservation by 5 times or more.