公开(公告)号：US09732371B2

公开(公告)日：2017-08-15

申请号：US14036339

申请日：2013-09-25

Applicant: EMD Millipore Corporation

Inventor： Gael Waiche ,  Monika Kukuczka

IPC: C12Q1/24 ,  C12M1/30 ,  C12M1/26

CPC classification number: C12Q1/24 ,  C12M33/02 ,  C12M33/14

Abstract: A method for transferring a filter membrane (130) bearing a retentate to a reagent pad (310), in which the membrane is mounted in a support frame (120) having a shoulder (121) and a skirt (122) projecting from the shoulder, the pad being mounted on a cassette (300) of complementary form to said skirt, the skirt being adapted to slide sealingly on the cassette until it comes into abutment formed by the shoulder, the method comprising the steps of sealingly engaging the skirt of the membrane support frame on the cassette, establishing a pressure difference between the cassette and the membrane support frame such that the membrane adopts the shape of a dome pointing towards the pad, while maintaining the pressure difference, applying a force on the frame so as to make the skirt slide on the cassette until contact is established between the end of the dome and the pad, maintaining a force on the frame so as to move it downward until it comes into abutment formed by the shoulder while maintaining the pressure difference.

公开(公告)号：US20140017779A1

公开(公告)日：2014-01-16

申请号：US14036339

申请日：2013-09-25

Applicant: EMD Millipore Corporation

Inventor： Gael Waiche ,  Monika Kukuczka

IPC: C12Q1/24

CPC classification number: C12Q1/24 ,  C12M33/02 ,  C12M33/14

Abstract: A method for transferring a filter membrane (130) bearing a retentate to a reagent pad (310), in which the membrane is mounted in a support frame (120) having a shoulder (121) and a skirt (122) projecting from the shoulder, the pad being mounted on a cassette (300) of complementary form to said skirt, the skirt being adapted to slide sealingly on the cassette until it comes into abutment formed by the shoulder, the method comprising the steps of sealingly engaging the skirt of the membrane support frame on the cassette, establishing a pressure difference between the cassette and the membrane support frame such that the membrane adopts the shape of a dome pointing towards the pad, while maintaining the pressure difference, applying a force on the frame so as to make the skirt slide on the cassette until contact is established between the end of the dome and the pad, maintaining a force on the frame so as to move it downward until it comes into abutment formed by the shoulder while maintaining the pressure difference.

Abstract translation: 一种用于将带有滞留物的过滤膜（130）转移到试剂垫（310）的方法，其中所述膜安装在具有肩部（121）的支撑框架（120）和从肩部突出的裙部（122） ，所述垫被安装在所述裙部的互补形式的盒（300）上，所述裙部适于在所述盒子上密封地滑动，直到其进入与所述肩部形成的邻接，所述方法包括以下步骤：密封地接合所述裙部的裙部 膜盒支撑框架，在盒和膜支撑框架之间形成压力差，使得膜采取指向垫的圆顶形状，同时保持压力差，对框架施加力，以使 裙子在盒子上滑动，直到在圆顶和垫子的端部之间建立接触，保持框架上的力，以向下移动它，直到它与肩部形成邻接 压力差。