公开(公告)号：US20220186259A1

公开(公告)日：2022-06-16

申请号：US17605190

申请日：2020-06-25

Applicant: EMD Millipore Corporation

Inventor： Naohisa Yoshioka

IPC: C12N15/86 ,  C12N9/12

Abstract: Synthetic, self-replicating RNA vectors comprising sequence encoding at least one immortalization protein, and use of the self-replicating RNA vectors to extend the lifespan of primary cell populations and expand said populations of primary cells.

公开(公告)号：US20220186235A1

公开(公告)日：2022-06-16

申请号：US17606644

申请日：2020-05-13

Applicant: EMD Millipore Corporation

Inventor： Naohisa Yoshioka

IPC: C12N15/79 ,  C12N15/10 ,  C12N9/22 ,  C12N15/11

Abstract: Synthetic, noninfectious, self-replicating RNA vectors that encode CRISPR proteins are provided. Each self-replicating RNA vector comprises a sequence encoding a plurality of non-structural replication complex proteins from an alphavirus and a sequence encoding a CRISPR protein. Also provided are methods for genome editing in which a synthetic self-replicating RNA vector is transfected into cells along with at least one corresponding guide RNA.