公开(公告)号：US20220282211A1

公开(公告)日：2022-09-08

申请号：US17746097

申请日：2022-05-17

Applicant: EMULATE, INC.

Inventor： Alicia Stark ,  Brar Gurpreet

IPC: C12N5/0781 ,  C12M3/06 ,  C07K16/00

Abstract: The present invention relates to fluidic systems for producing IgG antibodies from co-cultures of white blood cells. In some embodiments, a microfluidic device containing co-cultures of an autologous whole peripheral white blood cell population including B cells, are used for providing antigen specific IgG antibody production from differentiating B cells (plasma cells). More specifically, high levels of IgM and IgG classes of antibodies are harvested from fluids flowing through the device. In some embodiments, IgG is produced during activation in the presence of antigen, including but not limited to therapeutic immunogenic compounds, e.g. engineered antibodies, vaccines, etc. In some embodiments, such co-cultures are further exposed to drug compounds e.g. for preclinical safety testing and individualized personal drug responses. In some embodiments, such antibody producing microfluidic devices are contemplated for use in companion diagnostic and complementary assays.